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  • Thermophiles from Deep Subsurface Waters

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    Ogg_2011_02Thesis.pdf (24.05Mb)
    Author(s)
    Ogg, Christopher D.
    Primary Supervisor
    Patel, Bharat
    Other Supervisors
    Greene, Tony
    Year published
    2011
    Metadata
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    Abstract
    Thermophiles dominate deep subsurface aquifers and represent the most ancient life on the planet. In this project, the culturable diversity of thermophiles that inhabit the world’s largest freshwater geothermal aquifer, Australia’s Great Artesian Basin (GAB), were explored using culture-dependent techniques. These studies largely focused on four unique microbial mat communities that exist in the runoff channel of the New Lorne Bore (registered number 17263) at temperatures between 52 – 72 °C and mostly targeted the cultivation of iron(III)- reducing bacteria due to their considerable influence on subsurface processes and ...
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    Thermophiles dominate deep subsurface aquifers and represent the most ancient life on the planet. In this project, the culturable diversity of thermophiles that inhabit the world’s largest freshwater geothermal aquifer, Australia’s Great Artesian Basin (GAB), were explored using culture-dependent techniques. These studies largely focused on four unique microbial mat communities that exist in the runoff channel of the New Lorne Bore (registered number 17263) at temperatures between 52 – 72 °C and mostly targeted the cultivation of iron(III)- reducing bacteria due to their considerable influence on subsurface processes and likely involvement in the corrosion of GAB bores and pipelines. During this project a number of novel and modified screening methods were developed including high-throughput microenrichments of thermophiles using Biolog (Biolog Inc., U.S.A.) and U-bottom deep-well (1 ml) microtiter plates (Sarstedt, Germany) amended with seldom tested energy substrates and varied terminal electron acceptors (TEA) such as iron(III), sulphate, vanadium(V) and molybdenum(VI). Phylogenetic analyses of the isolates recovered in this project detected more than 15 novel phylotypes thereby extending the known culturable diversity of the GAB microflora. Complete phenotypic characterisations were performed on 9 GAB thermoanaerobic isolates, which resulted in the descriptions of 8 novel organisms including 4 novel genera: Thermotalea metallivorans (Ogg & Patel, 2009b), Fervidicola ferrireducens (Ogg & Patel, 2009c), Sporolituus thermophilus (Ogg & Patel, 2009d) and Fervidicella metallireducens (Ogg & Patel, 2010); and 4 novel species: Caloramator australicus (Ogg & Patel, 2009a), Caloramator mitchellensis (Ogg & Patel, 2011a), Thermovenabulum gondwanense (Ogg et al., 2010), and Desulfotomaculum varum (Ogg & Patel, 2011c); and amendments to the genus Caloramator (Ogg & Patel, 2011a) [given in Appendices 1 – 8]. Included in these characterisation studies was a modified method for determining an organisms mol % of G + C content of DNA using TempliPhi (Amersham Biosciences, U.K.) genome amplification kits to prepare the high molecular weight (HMW) genomic DNA prior to thermal denaturation. When the phenotypic data resulting from the strain characterisations was combined with the enrichment results, a complex model of the microbial oxidation of organic matter in the GAB at elevated temperatures (50 – 70 °C) was constructed.
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    Thesis Type
    Thesis (PhD Doctorate)
    Degree Program
    Doctor of Philosophy (PhD)
    School
    School of Biomolecular and Physical Sciences
    DOI
    https://doi.org/10.25904/1912/2721
    Copyright Statement
    The author owns the copyright in this thesis, unless stated otherwise.
    Item Access Status
    Public
    Note
    Commercially published articles included in Appendices have not been published here.
    Subject
    Thermophiles
    New Lorne Bore
    Australia's Great artesian Basin
    Thermotalea metallivorans
    Fervidicola ferrireducens
    Sporolituus thermophilus
    Fervidicella metallireducens
    Caloramator australicus
    Caloramator mitchellensis
    Thermovenabulum gondwanense
    Desulfotomaculum varum
    Publication URI
    http://hdl.handle.net/10072/366568
    Collection
    • Theses - Higher Degree by Research

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