Characterisation of Campylobacter jejuni Lipooligosaccharides
MetadataShow full item record
Campylobacter jejuni is a major bacterial cause of food-borne enteritis, and its lipooligosaccharide (LOS) plays an initiating role in the development of the autoimmune neuropathy, Guillain-Barré syndrome, by induction of anti-neural cross-reactive antibodies through ganglioside molecular mimicry. Herein we describe the existence and heterogeneity of multiple LOS forms in C. jejuni strains of human and chicken origin grown at 37°C and 42°C. The C. jejuni NCTC 11168 original isolate (11168-O) was compared to the genome-sequenced variant (11168-GS), and both were found to have a lower-Mr LOS form, which was different in size and structure to the previously characterized higher-Mr form bearing GM1 mimicry. The lower-Mr form production was found to be dependent on the growth temperature as the production of this form increased from ~5 %, observed at 37°C to ~35 % at 42°C. The structure of the lower-Mr form contained a Galβ1,3GalNAc disaccharide moiety which is consistent with the termini of the GM1, asialo-GM1, GD1, GT1 and GQ1 gangliosides, however, it did not display GM1 mimicry as assessed in blotting studies but was shown by NMR analysis to resemble asialo-GM1. The production of multiple LOS forms and lack of GM1 mimicry was not a result of phase variation in the genes tested for NCTC 11168 and was also observed in most of the human and chicken isolates of C. jejuni tested.
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
Institute for Glycomics
Item Access Status
The Appendices I to L on pages 150-153 have not been published for copyright reasons.