Structural and Functional Comparisons of Human and Zebrafish Galectin-1
Author(s)
Primary Supervisor
Ralph, Stephen
Other Supervisors
Day, Christopher
Year published
2016
Metadata
Show full item recordAbstract
Galectin-1 (Gal-1) is canonically referred to as a ‘galactose-binding lectin’ with several mportant functional and structural characteristics in the immune system. As a target for pharmaceutical research that is gaining in its significance for cancer biology, it is surprising that studies have not focused on Gal-1 structures outside of the mammalian taxa. For this reason, a recombinant Zebrafish Galectin-1 (zGal-1) was utilised as a novel homologous model for hGal-1. On determining the zGal-1 3D structure, several insights were gained through a range of biochemical, biophysical and computational techniques.
The results ...
View more >Galectin-1 (Gal-1) is canonically referred to as a ‘galactose-binding lectin’ with several mportant functional and structural characteristics in the immune system. As a target for pharmaceutical research that is gaining in its significance for cancer biology, it is surprising that studies have not focused on Gal-1 structures outside of the mammalian taxa. For this reason, a recombinant Zebrafish Galectin-1 (zGal-1) was utilised as a novel homologous model for hGal-1. On determining the zGal-1 3D structure, several insights were gained through a range of biochemical, biophysical and computational techniques. The results showed similarities between structural characteristics of the reduced forms of hGal-1 and zGal-1. A collection of glycan array data by in-house analysis and analysis of internet databases was also undertaken to evaluate the similarities and differences across vertebrate Galectins. Gal-1 binding glycan array profiles indicated its strong panhomologous affinity for numerous fucosylated and sulphated glycans.
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View more >Galectin-1 (Gal-1) is canonically referred to as a ‘galactose-binding lectin’ with several mportant functional and structural characteristics in the immune system. As a target for pharmaceutical research that is gaining in its significance for cancer biology, it is surprising that studies have not focused on Gal-1 structures outside of the mammalian taxa. For this reason, a recombinant Zebrafish Galectin-1 (zGal-1) was utilised as a novel homologous model for hGal-1. On determining the zGal-1 3D structure, several insights were gained through a range of biochemical, biophysical and computational techniques. The results showed similarities between structural characteristics of the reduced forms of hGal-1 and zGal-1. A collection of glycan array data by in-house analysis and analysis of internet databases was also undertaken to evaluate the similarities and differences across vertebrate Galectins. Gal-1 binding glycan array profiles indicated its strong panhomologous affinity for numerous fucosylated and sulphated glycans.
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Thesis Type
Thesis (PhD Doctorate)
Degree Program
Doctor of Philosophy (PhD)
School
School of Medical Science
Copyright Statement
The author owns the copyright in this thesis, unless stated otherwise.
Subject
Human Galectin-1
Zebrafish Galectin-1
Galectin-1 (Gal-1)
Galactose-binding lectin
Cancer biology