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  • The Development of a Real-Time Quantitative Polymerase Chain Reaction Method for the Diagnosis of Staphylococcus Aureus in Intravascular Catheter Colonisation

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    Higgins,Maddie_Final thesis_Redacted.pdf (2.109Mb)
    Author(s)
    Higgins, Maddie Joyce
    Primary Supervisor
    Ford, Rebecca
    Zhang, Li
    Year published
    2017
    Metadata
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    Abstract
    Staphylococcus aureus (S. aureus) catheter-related bloodstream infection (CRBSI) remains a leading cause of nosocomial (hospital acquired) infection worldwide. Early detection of S. aureus as a causative organism and appropriate antibiotic therapy is crucial in reducing patient morbidity and mortality rates. Current diagnosis of S. aureus intravascular catheter (IVC) colonisation relies on the use of conventional culture-dependent methods. However, many fastidious bacteria are difficult to be recovered in current laboratory conditions. Furthermore, a definitive diagnosis following a positive culture may take up to seven days ...
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    Staphylococcus aureus (S. aureus) catheter-related bloodstream infection (CRBSI) remains a leading cause of nosocomial (hospital acquired) infection worldwide. Early detection of S. aureus as a causative organism and appropriate antibiotic therapy is crucial in reducing patient morbidity and mortality rates. Current diagnosis of S. aureus intravascular catheter (IVC) colonisation relies on the use of conventional culture-dependent methods. However, many fastidious bacteria are difficult to be recovered in current laboratory conditions. Furthermore, a definitive diagnosis following a positive culture may take up to seven days and is therefore not useful in directing early antibiotic therapy in patients with CRBSI. A real-time polymerase chain reaction (PCR) assay, using primers specific to the thermonuclease (nuc) gene, targeting the S. aureus genome has the potential to address these limitations.
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    Thesis Type
    Thesis (Masters)
    Degree Program
    Master of Science (MSc)
    School
    School of Natural Sciences
    DOI
    https://doi.org/10.25904/1912/2801
    Copyright Statement
    The author owns the copyright in this thesis, unless stated otherwise.
    Subject
    Staphylococcus aureus (S. aureus)
    Catheter-related bloodstream infection (CRBSI)
    Nosocomial (hospital acquired) infection
    Polymerase chain reaction (PCR) assay
    Intravascular catheter colonisation
    Publication URI
    http://hdl.handle.net/10072/367624
    Collection
    • Theses - Higher Degree by Research

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