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dc.contributor.advisorTonissen, Kathryn
dc.contributor.authorHawkes, Hye-Jin
dc.date.accessioned2018-01-23T02:55:24Z
dc.date.available2018-01-23T02:55:24Z
dc.date.issued2011
dc.identifier.doi10.25904/1912/1584
dc.identifier.urihttp://hdl.handle.net/10072/367671
dc.description.abstractWhen cells are exposed to oxidative stress reactive oxygen species (ROS) are formed, causing severe damage in the cell. To maintain the redox homeostasis, antioxidant systems are switched on. Two of the major redox proteins include thioredoxin (Trx) and Methionine Sulfoxide Reductase (MSR). Trx interacts directly and indirectly with several transcription factors to modulate binding to gene promoters. In particular, Nrf2 is known to be an important transcription factor in redox regulation. To further define the mechanisms by which the Trx promoter is regulated by oxidative stress, the HEC1B endometrial cancer cell line and MDA-­‐MB-­‐231 breast cancer cell line were transfected with various Trx gene promoter constructs that drive expression of a luciferase reporter. When cells were transfected with constructs containing the Trx gene promoter region where the Antioxidant Responsive Element (ARE) was deleted, no induction was observed in response to tBHQ, diamide or H2O2 utilising luciferase reporter activity assays. Thus, the results confirmed the published data that the ARE is one of the most important elements in activating the Trx gene promoter during oxidative stress. Furthermore, it was established that luciferase reporter assays using the same cell line grown in media supplemented with different serum yielded significantly different results. Cells grown in medium supplemented with the serum containing the highest selenium level resulted in the lowest fold induction of the Trx gene promoter, while sera with the lowest level resulted in the highest folld inductions.
dc.languageEnglish
dc.publisherGriffith University
dc.publisher.placeBrisbane
dc.rights.copyrightThe author owns the copyright in this thesis, unless stated otherwise.
dc.subject.keywordsReactive oxygen species
dc.subject.keywordsThioredoxin
dc.subject.keywordsMethionione sulfoxide reductase
dc.subject.keywordsRedox gene promoters
dc.subject.keywordsReductase A
dc.titleAnalysis of Redox Gene Promoters and Structural Mechanisms, Focusing on Thioredoxin and Methionine Sulfoxide Reductase A
dc.typeGriffith thesis
gro.facultyScience, Environment, Engineering and Technology
gro.rights.copyrightThe author owns the copyright in this thesis, unless stated otherwise.
gro.hasfulltextFull Text
dc.contributor.otheradvisorClarke, Frank
dc.contributor.otheradvisorHwang, Kwang Yeon
dc.rights.accessRightsPublic
gro.identifier.gurtIDgu1342505139996
gro.source.ADTshelfnoADT0
gro.source.GURTshelfnoGURT1257
gro.thesis.degreelevelThesis (PhD Doctorate)
gro.thesis.degreeprogramDoctor of Philosophy (PhD)
gro.departmentSchool of Biomolecular and Physical Sciences
gro.griffith.authorHawkes, Hye-Jin


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