Decellularized Tissue Engineered Constructs Using Cell Sheet Technology

View/ Open
Author(s)
Primary Supervisor
Ivanovski, Saso
Other Supervisors
Hamlet, Stephen
Hutmacher, Dietmar
Hashimi, Saeed
Year published
2016
Metadata
Show full item recordAbstract
The periodontium is a complex structure that is comprised of soft tissue components the gingiva and periodontal ligament, as well as hard tissue components in the form of alveolar bone and cementum. Human periodontal ligament cells (HPDLCs) and their extracellular matrix are regarded as essential components to achieve successful periodontal regeneration when treating periodontal lesions.
In the present study, cell sheet technology was utilized to fabricate periodontal ligament cell sheets. These cell sheets were subsequently decellularized to isolate and preserve the extracellular component structural and functional ...
View more >The periodontium is a complex structure that is comprised of soft tissue components the gingiva and periodontal ligament, as well as hard tissue components in the form of alveolar bone and cementum. Human periodontal ligament cells (HPDLCs) and their extracellular matrix are regarded as essential components to achieve successful periodontal regeneration when treating periodontal lesions. In the present study, cell sheet technology was utilized to fabricate periodontal ligament cell sheets. These cell sheets were subsequently decellularized to isolate and preserve the extracellular component structural and functional characteristics, and were evaluated in vitro and in vivo. In all experiments that were undertaken in this study, melt electro-spun polycaprolactone (PCL) scaffolds were used as a carrier for the cell sheets, in order to support their fragile nature during the processes of decellularization. The combined cell sheet-PCL scaffold structure is referred to as a decellularized cell sheet construct.
View less >
View more >The periodontium is a complex structure that is comprised of soft tissue components the gingiva and periodontal ligament, as well as hard tissue components in the form of alveolar bone and cementum. Human periodontal ligament cells (HPDLCs) and their extracellular matrix are regarded as essential components to achieve successful periodontal regeneration when treating periodontal lesions. In the present study, cell sheet technology was utilized to fabricate periodontal ligament cell sheets. These cell sheets were subsequently decellularized to isolate and preserve the extracellular component structural and functional characteristics, and were evaluated in vitro and in vivo. In all experiments that were undertaken in this study, melt electro-spun polycaprolactone (PCL) scaffolds were used as a carrier for the cell sheets, in order to support their fragile nature during the processes of decellularization. The combined cell sheet-PCL scaffold structure is referred to as a decellularized cell sheet construct.
View less >
Thesis Type
Thesis (PhD Doctorate)
Degree Program
Doctor of Philosophy (PhD)
School
School of Dentistry and Oral Health
Copyright Statement
The author owns the copyright in this thesis, unless stated otherwise.
Item Access Status
Public
Subject
Periodontium
gingiva ligament
periodontal ligament
Alveolar bone
Human periodontal ligament cells (HPDLCs)
Cell sheet-PCL scaffold structure