Show simple item record

dc.contributor.authorMaroof, Hamidreza
dc.contributor.authorIslam, Farhadul
dc.contributor.authorAriana, Armin
dc.contributor.authorGopalan, Vinod
dc.contributor.authorLam, Alfred K
dc.date.accessioned2018-02-22T12:00:19Z
dc.date.available2018-02-22T12:00:19Z
dc.date.issued2017
dc.identifier.issn1355-008X
dc.identifier.doi10.1007/s12020-017-1393-3
dc.identifier.urihttp://hdl.handle.net/10072/368777
dc.description.abstractPurpose: This study aims to determine the expression of miR-34b-5p in thyroid carcinomas and to investigate the role of miR34b-5p in the modulation of proteins involved in angiogenesis of thyroid carcinoma cells. Methods: The expressions of miR-34b-5p levels in five cell lines and 65 tissue samples from thyroid carcinomas were examined by real-time polymerase chain reaction. An exogenous miR-34b-5p (mimic) transiently overexpress miR-34b-5p in theses thyroid carcinoma cells. The effects of miR-34b-5p overexpression on the proteins involved in angiogenesis and cell cycle regulations (VEGF-A, Bcl-2 and Notch1) were investigated by Western blot, immunofluorescence, enzyme-linked immunosorbent assay followed by cell cycle analysis and apoptosis assays. Results: miR-34b-5p is markedly downregulated in all thyroid carcinoma cell lines and tissues samples when compared with non-neoplastic immortalised thyroid cell line and non-neoplastic thyroid tissues, respectively. The expression levels of miR-34b were significantly associated with T-stages of thyroid carcinomas (p = 0.042). Downregulation of VEGF-A, Bcl-2 and Notch1 proteins in thyroid carcinoma cells were noted in cells that transiently transfected with miR-34b-5p mimic. In addition, enzyme-linked immunosorbent assay confirmed the decreased expression of VEGF in thyroid carcinoma cells after transfection with miR-34b-5p mimic. Furthermore, miR-34b-5p mimic transfection induces significant accumulation of cells in G0-G1 of the cell cycle by blocking of their entry into the S transitional phase as well as increasing the total apoptosis. Conclusions: miR-34b-5p functions as a potent regulator of angiogenesis, apoptosis and cell proliferation via modulation of VEGF-A, Bcl-2 and Notch1 proteins. It could be a target for developing treatment strategies of thyroid carcinoma with aggressive clinical behaviour.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofpagefrom153
dc.relation.ispartofpageto166
dc.relation.ispartofjournalEndocrine
dc.relation.ispartofvolume58
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchClinical sciences not elsewhere classified
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode320299
dc.titleThe roles of microRNA-34b-5p in angiogenesis of thyroid carcinoma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.facultyGriffith Health, School of Medicine
gro.rights.copyright© 2017 Springer US. This is an electronic version of an article published in Endocrine, Volume 58, Issue 1, pp 153–166, 2017. Endocrine is available online at: http://link.springer.com/ with the open URL of your article.
gro.hasfulltextFull Text
gro.griffith.authorLam, Alfred K.
gro.griffith.authorAriana, Armin S.
gro.griffith.authorGopalan, Vinod
gro.griffith.authorIslam, Farhad


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record