dc.contributor.author | Maroof, Hamidreza | |
dc.contributor.author | Islam, Farhadul | |
dc.contributor.author | Ariana, Armin | |
dc.contributor.author | Gopalan, Vinod | |
dc.contributor.author | Lam, Alfred K | |
dc.date.accessioned | 2018-02-22T12:00:19Z | |
dc.date.available | 2018-02-22T12:00:19Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1355-008X | |
dc.identifier.doi | 10.1007/s12020-017-1393-3 | |
dc.identifier.uri | http://hdl.handle.net/10072/368777 | |
dc.description.abstract | Purpose:
This study aims to determine the expression of miR-34b-5p in thyroid carcinomas and to investigate the role of miR34b-5p in the modulation of proteins involved in angiogenesis of thyroid carcinoma cells.
Methods:
The expressions of miR-34b-5p levels in five cell lines and 65 tissue samples from thyroid carcinomas were examined by real-time polymerase chain reaction. An exogenous miR-34b-5p (mimic) transiently overexpress miR-34b-5p in theses thyroid carcinoma cells. The effects of miR-34b-5p overexpression on the proteins involved in angiogenesis and cell cycle regulations (VEGF-A, Bcl-2 and Notch1) were investigated by Western blot, immunofluorescence, enzyme-linked immunosorbent assay followed by cell cycle analysis and apoptosis assays.
Results:
miR-34b-5p is markedly downregulated in all thyroid carcinoma cell lines and tissues samples when compared with non-neoplastic immortalised thyroid cell line and non-neoplastic thyroid tissues, respectively. The expression levels of miR-34b were significantly associated with T-stages of thyroid carcinomas (p = 0.042). Downregulation of VEGF-A, Bcl-2 and Notch1 proteins in thyroid carcinoma cells were noted in cells that transiently transfected with miR-34b-5p mimic. In addition, enzyme-linked immunosorbent assay confirmed the decreased expression of VEGF in thyroid carcinoma cells after transfection with miR-34b-5p mimic. Furthermore, miR-34b-5p mimic transfection induces significant accumulation of cells in G0-G1 of the cell cycle by blocking of their entry into the S transitional phase as well as increasing the total apoptosis.
Conclusions:
miR-34b-5p functions as a potent regulator of angiogenesis, apoptosis and cell proliferation via modulation of VEGF-A, Bcl-2 and Notch1 proteins. It could be a target for developing treatment strategies of thyroid carcinoma with aggressive clinical behaviour. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Springer | |
dc.relation.ispartofpagefrom | 153 | |
dc.relation.ispartofpageto | 166 | |
dc.relation.ispartofjournal | Endocrine | |
dc.relation.ispartofvolume | 58 | |
dc.subject.fieldofresearch | Clinical sciences | |
dc.subject.fieldofresearch | Clinical sciences not elsewhere classified | |
dc.subject.fieldofresearchcode | 3202 | |
dc.subject.fieldofresearchcode | 320299 | |
dc.title | The roles of microRNA-34b-5p in angiogenesis of thyroid carcinoma | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dc.description.version | Accepted Manuscript (AM) | |
gro.faculty | Griffith Health, School of Medicine | |
gro.rights.copyright | © 2017 Springer US. This is an electronic version of an article published in Endocrine, Volume 58, Issue 1, pp 153–166, 2017. Endocrine is available online at: http://link.springer.com/ with the open URL of your article. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Lam, Alfred K. | |
gro.griffith.author | Ariana, Armin S. | |
gro.griffith.author | Gopalan, Vinod | |
gro.griffith.author | Islam, Farhad | |