dc.contributor.author | Catterall, Kylie | |
dc.contributor.author | Robertson, David | |
dc.contributor.author | Teasdale, Peter R | |
dc.contributor.author | Welsh, David T | |
dc.contributor.author | John, Richard | |
dc.date.accessioned | 2017-05-03T11:47:20Z | |
dc.date.available | 2017-05-03T11:47:20Z | |
dc.date.issued | 2010 | |
dc.date.modified | 2011-03-07T08:54:42Z | |
dc.identifier.issn | 0039-9140 | |
dc.identifier.doi | 10.1016/j.talanta.2009.10.057 | |
dc.identifier.uri | http://hdl.handle.net/10072/36900 | |
dc.description.abstract | A number of recent studies have utilised ferricyanide as a respiratory mediator for microbial-based assays for determining water quality parameters such as biochemical oxygen demand (BOD) and toxicity. The majority of assays published to date obtain a result by determining the difference in ferrocyanide accumulation between a test sample and one or more control samples. However, a validation of the relationship between ferrocyanide accumulation and standard measures of cell density or viability has not yet been performed. To this end, a rapid microbially catalysed ferricyanide-mediated respiration (FM-RES) assay was compared with standard plate count (SPC) and spectrophotometer (OD600) measurements on a growing batch culture of Escherichia coli. Good agreement was observed between all techniques, with predictable deviations noted in different phases of the growth curves. Standardised FM-RES assays showed excellent correlations with the SPC method under controlled conditions, indicating that short-term changes in microbial activity are due to a change in per-cell respiration, rather than changes in cell numbers. The FM-RES assay was then used to observe the changes in the respiration of E. coli induced by the addition of a glucose-glutamic acid (GGA) mixture, 3,5-dichlorophenol (3,5-DCP) and Ag+ in various combinations and concentrations. Stimulation of respiration was pronounced in the presence of GGA while both 3,5-DCP and, in particular, Ag+ demonstrated inhibitory respiratory effects. The results highlight the validity and suitability of ferricyanide-mediated respiration bioassays, with appropriate modification, to monitor either stimulatory effects on microbial populations, such as occurs with BOD, or inhibitory effects, such as occurs with toxicity assays. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.publisher.place | Netherlands | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 1980 | |
dc.relation.ispartofpageto | 1985 | |
dc.relation.ispartofissue | 5 | |
dc.relation.ispartofjournal | Talanta | |
dc.relation.ispartofvolume | 80 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Analytical chemistry | |
dc.subject.fieldofresearch | Analytical chemistry not elsewhere classified | |
dc.subject.fieldofresearch | Other chemical sciences | |
dc.subject.fieldofresearchcode | 3401 | |
dc.subject.fieldofresearchcode | 340199 | |
dc.subject.fieldofresearchcode | 3499 | |
dc.title | Evaluating use of ferricyanide-mediated respiration bioassays to quantify stimulatory and inhibitory effects on Escherichia coli populations | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.faculty | Griffith Sciences, Griffith School of Environment | |
gro.date.issued | 2010 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | John, Richard | |