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dc.contributor.authorShamel, Mohamed
dc.contributor.authorAl Ankily, Mahmoud M.
dc.contributor.authorBakr, Mahmoud
dc.date.accessioned2019-04-07T23:21:09Z
dc.date.available2019-04-07T23:21:09Z
dc.date.issued2017
dc.identifier.issn2574-271X
dc.identifier.doi10.33140/SCRI
dc.identifier.urihttp://hdl.handle.net/10072/369028
dc.description.abstractStem cells are characterized by the ability to form clones, self-regenerate and differentiate into different types of cells. Stem cells derived from human dental pulp (DPSCs) have been shown to differentiate into odontoblasts in vitro after using Bone Morphogenic Protein -2 (BMP-2). The aim of the present study was to explore the variations in the differentiation potential of the DPSC into odontoblasts in diabetic adults; it also aimed at investigating the possible influence of the origin of stem cells (coronal and radicular pulp) on their differentiation potential. Pulp tissue was extirpated from healthy individuals as well as from controlled and uncontrolled diabetic patients. Pulp tissue was divided into coronal and radicular parts and each were cultured for 30 days and then BMP-2 was added. Alizarin red staining was performed to confirm mineralization. RT-PCR was used to analyse expression of mineralization markers Dentin sialophosphoprotein (DSPP) & Enamelysin. The results of this study showed that DPSCs of both healthy and diabetic groups had stem cell properties. DPSCs of the coronal pulp have a more proliferative capacity than that of the radicular pulp. Controlled diabetes mellitus provides relatively unfavourable conditions for the DPSCs to proliferate and differentiate while uncontrolled diabetes produces more deleterious effect on the DPSCs capacity for proliferation and differentiation. Expression of DSPP and enamelysin was positive in healthy and controlled diabetics groups, while in the uncontrolled diabetic group it was negative. It is concluded that DPSCs of the coronal pulp have a more proliferative capacity than that of the radicular pulp, controlled diabetes mellitus provides relatively unfavourable conditions for the DPSCs to proliferate and differentiate and uncontrolled diabetes produces more deleterious effect on the DPSCs capacity for proliferation and differentiation.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherOpast Group
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto6
dc.relation.ispartofissue3
dc.relation.ispartofjournalJournal of Stem Cell Research
dc.relation.ispartofvolume1
dc.subject.fieldofresearchDental Therapeutics, Pharmacology and Toxicology
dc.subject.fieldofresearchOral Medicine and Pathology
dc.subject.fieldofresearchcode110502
dc.subject.fieldofresearchcode110505
dc.titleProliferative Capacity and Differentiation Potential of Isolated Postnatal Human Dental Pulp Stem Cells in Diabetic Patients
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorBakr, Mahmoud


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