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dc.contributor.authorA. Apicella, Michaelen_US
dc.contributor.authorM. B. Post, Deborahen_US
dc.contributor.authorC. Fowler, Andrewen_US
dc.contributor.authorD. Jones, Bradleyen_US
dc.contributor.authorA. Rasmussen, Jeden_US
dc.contributor.authorR. Hunt, Jasonen_US
dc.contributor.authorImagawa, Sayakaen_US
dc.contributor.authorChoudhury, Biswaen_US
dc.contributor.authorJ. Inzana, Thomasen_US
dc.contributor.authorM. Maier, Tamaraen_US
dc.contributor.authorW. Frank, Daraen_US
dc.contributor.authorC. Zahrt, Thomasen_US
dc.contributor.authorChaloner, Kathrynen_US
dc.contributor.authorJennings, Michaelen_US
dc.contributor.authorK. McLendon, Mollyen_US
dc.contributor.authorW. Gibson, Bradforden_US
dc.date.accessioned2017-04-24T08:43:11Z
dc.date.available2017-04-24T08:43:11Z
dc.date.issued2010en_US
dc.date.modified2011-03-18T07:02:40Z
dc.identifier.issn19326203en_US
dc.identifier.doi10.1371/journal.pone.0011060en_AU
dc.identifier.urihttp://hdl.handle.net/10072/36904
dc.description.abstractCapsular polysaccharides are important factors in bacterial pathogenesis and have been the target of a number of successful vaccines. Francisella tularensis has been considered to express a capsular antigen but none has been isolated or characterized. We have developed a monoclonal antibody, 11B7, which recognizes the capsular polysaccharide of F. tularensis migrating on Western blot as a diffuse band between 100 kDa and 250 kDa. The capsule stains poorly on SDS-PAGE with silver stain but can be visualized using ProQ Emerald glycoprotein stain. The capsule appears to be highly conserved among strains of F. tularensis as antibody 11B7 bound to the capsule of 14 of 14 F. tularensis type A and B strains on Western blot. The capsular material can be isolated essentially free of LPS, is phenol and proteinase K resistant, ethanol precipitable and does not dissociate in sodium dodecyl sulfate. Immunoelectron microscopy with colloidal gold demonstrates 11B7 circumferentially staining the surface of F. tularensis which is typical of a polysaccharide capsule. Mass spectrometry, compositional analysis and NMR indicate that the capsule is composed of a polymer of the tetrasaccharide repeat, 4)-a-D-GalNAcAN-(1->4)-a-D-GalNAcAN-(1->?3)-߭D-QuiNAc-(1->2)-߭D-Qui4NFm-(1-,which is identical to the previously described F. tularensis O-antigen subunit. This indicates that the F. tularensis capsule can be classified as an O-antigen capsular polysaccharide. Our studies indicate that F. tularensis O-antigen glycosyltransferase mutants do not make a capsule. An F. tularensis acyltransferase and an O-antigen polymerase mutant had no evidence of an O-antigen but expressed a capsular antigen. Passive immunization of BALB/c mice with 75 姠of 11B7 protected against a 150 fold lethal challenge of F. tularensis LVS. Active immunization of BALB/c mice with 10 姠of capsule showed a similar level of protection. These studies demonstrate that F. tularensis produces an O-antigen capsule that may be the basis of a future vaccineen_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.format.extent2490708 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherPublic Library of Scienceen_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom1en_US
dc.relation.ispartofpageto18en_US
dc.relation.ispartofissue7en_US
dc.relation.ispartofjournalPloS Oneen_US
dc.relation.ispartofvolume5en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchMedical and Health Sciences not elsewhere classifieden_US
dc.subject.fieldofresearchcode119999en_US
dc.titleIdentification, Characterization and Immunogenicity of an O-Antigen Capsular Polysaccharide of Francisella tularensisen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright 2010 Apicella et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)en_AU
gro.date.issued2010
gro.hasfulltextFull Text


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