Biotechnological Improvements of Cold-Adapted Enzymes: Commercialization via an Integrated Approach
Author(s)
Siddiqui, Khawar Sohail
Shemsi, Ahsan Mushir
Guerriero, Gea
Najnin, Tahria
Taha
Ertan, Haluk
Griffith University Author(s)
Year published
2017
Metadata
Show full item recordAbstract
Cold-adapted (psychrophilic) enzymes have intrinsically high activities at the expense of low stabilities due to their flexible structures. Their higher thermolability limits their applications under numerous industrial conditions that require the process to be carried out at higher temperatures for efficient catalysis. Therefore, for effective utilization, cold-adapted enzymes need to be improved in such a way that enhances their stability with an increase or retention of their activity. This chapter discusses the thermodynamic aspects of improvement of catalytic properties and presents a unified strategy that aims at ...
View more >Cold-adapted (psychrophilic) enzymes have intrinsically high activities at the expense of low stabilities due to their flexible structures. Their higher thermolability limits their applications under numerous industrial conditions that require the process to be carried out at higher temperatures for efficient catalysis. Therefore, for effective utilization, cold-adapted enzymes need to be improved in such a way that enhances their stability with an increase or retention of their activity. This chapter discusses the thermodynamic aspects of improvement of catalytic properties and presents a unified strategy that aims at simultaneously improving the activity and stability of cold-adapted enzymes by employing not a single but a combination of approaches that include genetic and chemical modifications, immobilization, nonaqueous solvents, and additives. This concept aims to take cold-adapted enzymes a step further from current potential to cost-effective tangible commercial applications.
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View more >Cold-adapted (psychrophilic) enzymes have intrinsically high activities at the expense of low stabilities due to their flexible structures. Their higher thermolability limits their applications under numerous industrial conditions that require the process to be carried out at higher temperatures for efficient catalysis. Therefore, for effective utilization, cold-adapted enzymes need to be improved in such a way that enhances their stability with an increase or retention of their activity. This chapter discusses the thermodynamic aspects of improvement of catalytic properties and presents a unified strategy that aims at simultaneously improving the activity and stability of cold-adapted enzymes by employing not a single but a combination of approaches that include genetic and chemical modifications, immobilization, nonaqueous solvents, and additives. This concept aims to take cold-adapted enzymes a step further from current potential to cost-effective tangible commercial applications.
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Book Title
Psychrophiles: From Biodiversity to Biotechnology
Subject
Medical biotechnology not elsewhere classified