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dc.contributor.authorFaglin, Isabelleen_US
dc.contributor.authorWilson, Jennyen_US
dc.contributor.authorTiralongo, Joeen_US
dc.contributor.authorPeak, Ianen_US
dc.date.accessioned2017-05-03T11:47:37Z
dc.date.available2017-05-03T11:47:37Z
dc.date.issued2010en_US
dc.date.modified2012-02-10T02:23:08Z
dc.identifier.issn00086215en_US
dc.identifier.doi10.1016/j.carres.2010.07.042en_US
dc.identifier.urihttp://hdl.handle.net/10072/36921
dc.description.abstractThe genetic basis of lipo-oligosaccharide (LOS) biosynthesis for the bacterium Moraxella catarrhalis has been elucidated and functions suggested for each of the glycosyltransferases. In this study we have expressed and characterised one of these enzymes, the putative galactosyltransferase Lgt2B/C. The lgt2B/C gene was amplified from M. catarrhalis, expressed in Escherichia coli, and Lgt2B/C was purified. Analysis of its glycosyltransferase catalytic activity ascertained the pH and temperature optima. The donor specificity and acceptor specificity were examined and they showed that Lgt2B/C is a galactosyltransferase with relatively broad acceptor specificity with optimal activity in the presence of exogenous Mg2+.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom2151en_US
dc.relation.ispartofpageto2156en_US
dc.relation.ispartofissue15en_US
dc.relation.ispartofjournalCarbohydrate Researchen_US
dc.relation.ispartofvolume345en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchOrganic Chemistry not elsewhere classifieden_US
dc.subject.fieldofresearchcode030599en_US
dc.titleMoraxella catarrhalis Lgt2, a galactosyltransferase with broad acceptor substrate specificityen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomicsen_US
gro.date.issued2010
gro.hasfulltextNo Full Text


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