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  • Tuning the modular Paracoccus denitrificans respirome to adapt from aerobic respiration to anaerobic denitrification

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    GiannopoulosPUB2797.pdf (565.9Kb)
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    Author(s)
    Giannopoulos, Georgios
    Sullivan, Matthew J
    Hartop, Katherine R
    Rowley, Gary
    Gates, Andrew J
    Watmough, Nicholas J
    Richardson, David J
    Griffith University Author(s)
    Sullivan, Matthew J.
    Year published
    2017
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    Abstract
    Bacterial denitrification is a respiratory process that is a major source and sink of the potent greenhouse gas nitrous oxide. Many denitrifying bacteria can adjust to life in both oxic and anoxic environments through differential expression of their respiromes in response to environmental signals such as oxygen, nitrate and nitric oxide. We used steady-state oxic and anoxic chemostat cultures to demonstrate that the switch from aerobic to anaerobic metabolism is brought about by changes in the levels of expression of relatively few genes, but this is sufficient to adjust the configuration of the respirome to allow the ...
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    Bacterial denitrification is a respiratory process that is a major source and sink of the potent greenhouse gas nitrous oxide. Many denitrifying bacteria can adjust to life in both oxic and anoxic environments through differential expression of their respiromes in response to environmental signals such as oxygen, nitrate and nitric oxide. We used steady-state oxic and anoxic chemostat cultures to demonstrate that the switch from aerobic to anaerobic metabolism is brought about by changes in the levels of expression of relatively few genes, but this is sufficient to adjust the configuration of the respirome to allow the organism to efficiently respire nitrate without the significant release of intermediates, such as nitrous oxide. The regulation of the denitrification respirome in strains deficient in the transcription factors FnrP, Nnr and NarR was explored and revealed that these have both inducer and repressor activities, possibly due to competitive binding at similar DNA binding sites. This may contribute to the fine tuning of expression of the denitrification respirome and so adds to the understanding of the regulation of nitrous oxide emission by denitrifying bacteria in response to different environmental signals.
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    Journal Title
    Environmental Microbiology
    Volume
    19
    Issue
    12
    DOI
    https://doi.org/10.1111/1462-2920.13974
    Copyright Statement
    © 2017 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
    Subject
    Evolutionary biology
    Microbiology
    Microbiology not elsewhere classified
    Publication URI
    http://hdl.handle.net/10072/369508
    Collection
    • Journal articles

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