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dc.contributor.authorDu, Jinping
dc.contributor.authorRehm, Bernd HA
dc.date.accessioned2019-06-19T13:07:28Z
dc.date.available2019-06-19T13:07:28Z
dc.date.issued2018
dc.identifier.issn0141-5492
dc.identifier.doi10.1007/s10529-017-2473-4
dc.identifier.urihttp://hdl.handle.net/10072/369690
dc.description.abstractObjectives: To overcome laborious and costly procedures often associated with therapeutic protein production and purification, in vivo polyester immobilized sortase is explored for the production of human tumor necrosis factor alpha (TNFα) and human interferon alpha 2b (IFNα2b) by Escherichia coli. Results: Hybrid genes encoding PhaC-Sortase-TNFα or PhaC-Sortase-IFNα2b fusions (with a LPETG recognition signal immediately before TNFα or IFNα2b), mediated intracellular production of polyester (polyhydroxyalkanoate, PHA) beads in Escherichia coli. Upon isolation of respective PHA beads, pure soluble TNFα or IFNα2b was released by activating sortase via addition of CaCl2 and triglycine. TNFα and IFNα2b each were recognized by corresponding conformational antibodies in an ELISA assay. Conclusions: In vivo polyester immobilized sortase could be exploited for production and purification of high-value therapeutic proteins without laborious and costly downstream processing.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherSpringer Netherlands
dc.publisher.placeNetherlands
dc.relation.ispartofpagefrom369
dc.relation.ispartofpageto373
dc.relation.ispartofissue2
dc.relation.ispartofjournalBiotechnology Letters
dc.relation.ispartofvolume40
dc.subject.fieldofresearchBiological Sciences not elsewhere classified
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchTechnology
dc.subject.fieldofresearchcode069999
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode09
dc.subject.fieldofresearchcode10
dc.titlePurification of therapeutic proteins mediated by in vivo polyester immobilized sortase
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorRehm, Bernd


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