dc.contributor.author | Balinas, Cassandra | |
dc.contributor.author | Thao, Nguyen | |
dc.contributor.author | Johnston, Samantha | |
dc.contributor.author | Smith, Peter | |
dc.contributor.author | Staines, Donald | |
dc.contributor.author | Marshall-Gradisnik, Sonya | |
dc.date.accessioned | 2018-03-12T04:24:28Z | |
dc.date.available | 2018-03-12T04:24:28Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0125-877X | |
dc.identifier.doi | 10.12932/AP-200517-0086 | |
dc.identifier.uri | http://hdl.handle.net/10072/370529 | |
dc.description.abstract | Background: Viral infections and hypersensitivities are commonly reported by Chronic Fatigue Syndrome/Myalgic
Encephalomyelitis (CFS/ME) patients. Mast Cells (MC) uniquely mediate type 1 hypersensitivities and resolve viral
infections via toll-like receptor 3 (TLR3).
Objective: To characterise and compare mast cell progenitors (MCPs) in CFS/ME participants with a known MC
disorder, Systemic mastocytosis (SM), and secondly, to investigate the role of MC TLR3 in CFS/ME participants
following Polyinosinic:polycytidylic acid (Poly I:C) stimulation.
Methods: A total of 11 International Consensus Criteria defined CFS/ME participants (40.42 ± 10.31), 9 World Health
Organisation defined systemic mastocytosis (SM) participants (47.00 ± 10.37) and 12 healthy controls (HC) (36.36 ± 9.88)
were included. Following autoMACS magnetic separation, CD117+/LinMCPs
were stimulated with Poly I:C for 24hr.
MCP purity (CD117 and Lin2), maturity (CD34 and FcεRI), interaction receptors and ligands (CD154 and HLA-DR),
and SM-specific (CD2 and CD25) markers were measured using flow cytometry.
Results: There was a significant decrease in HLA-DR+/CD154-
expression between CFS/ME and SM groups pre and post
Poly I:C stimulation. There were no significant differences in maturity MCPs, CD154, and CD2/CD25 expression between
groups pre and post Poly I:C stimulation.
Conclusion: This pilot investigation provides a novel methodology to characterise MCPs in a rapid, inexpensive and
less invasive fashion. We report a significant decrease in HLA-DR+/CD154-
expression between CFS/ME and SM
participants, and an observed increase in HLA-DR/CD154+
expression post Poly I:C stimulation in CFS/ME
participants. Peripheral MCPs may be present in CFS/ME pathophysiology, however further investigation is required to
determine their immunological role. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Association of Allergy, Asthma and Immunology of Thailand (AAIAT) | |
dc.publisher.uri | http://apjai-journal.org/ | |
dc.relation.ispartofpagefrom | 1 | |
dc.relation.ispartofpageto | 8 | |
dc.relation.ispartofjournal | Asian Pacific Journal of Allergy and Immunology | |
dc.subject.fieldofresearch | Clinical sciences | |
dc.subject.fieldofresearch | Clinical sciences not elsewhere classified | |
dc.subject.fieldofresearch | Immunology | |
dc.subject.fieldofresearchcode | 3202 | |
dc.subject.fieldofresearchcode | 320299 | |
dc.subject.fieldofresearchcode | 3204 | |
dc.title | Investigation of mast cell toll-like receptor 3 in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and Systemic Mastocytosis participants using the novel application of autoMACS magnetic separation and flow cytometry | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dc.description.version | Version of Record (VoR) | |
gro.faculty | Griffith Health, School of Medical Science | |
gro.rights.copyright | © 2017 Asian Pacific Journal of Allergy and Immunology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Staines, Donald R. | |
gro.griffith.author | Marshall-Gradisnik, Sonya M. | |