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dc.contributor.authorBalinas, Cassandra
dc.contributor.authorThao, Nguyen
dc.contributor.authorJohnston, Samantha
dc.contributor.authorSmith, Peter
dc.contributor.authorStaines, Donald
dc.contributor.authorMarshall-Gradisnik, Sonya
dc.date.accessioned2018-03-12T04:24:28Z
dc.date.available2018-03-12T04:24:28Z
dc.date.issued2018
dc.identifier.issn0125-877X
dc.identifier.doi10.12932/AP-200517-0086
dc.identifier.urihttp://hdl.handle.net/10072/370529
dc.description.abstractBackground: Viral infections and hypersensitivities are commonly reported by Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) patients. Mast Cells (MC) uniquely mediate type 1 hypersensitivities and resolve viral infections via toll-like receptor 3 (TLR3). Objective: To characterise and compare mast cell progenitors (MCPs) in CFS/ME participants with a known MC disorder, Systemic mastocytosis (SM), and secondly, to investigate the role of MC TLR3 in CFS/ME participants following Polyinosinic:polycytidylic acid (Poly I:C) stimulation. Methods: A total of 11 International Consensus Criteria defined CFS/ME participants (40.42 ± 10.31), 9 World Health Organisation defined systemic mastocytosis (SM) participants (47.00 ± 10.37) and 12 healthy controls (HC) (36.36 ± 9.88) were included. Following autoMACS magnetic separation, CD117+/LinMCPs were stimulated with Poly I:C for 24hr. MCP purity (CD117 and Lin2), maturity (CD34 and FcεRI), interaction receptors and ligands (CD154 and HLA-DR), and SM-specific (CD2 and CD25) markers were measured using flow cytometry. Results: There was a significant decrease in HLA-DR+/CD154- expression between CFS/ME and SM groups pre and post Poly I:C stimulation. There were no significant differences in maturity MCPs, CD154, and CD2/CD25 expression between groups pre and post Poly I:C stimulation. Conclusion: This pilot investigation provides a novel methodology to characterise MCPs in a rapid, inexpensive and less invasive fashion. We report a significant decrease in HLA-DR+/CD154- expression between CFS/ME and SM participants, and an observed increase in HLA-DR/CD154+ expression post Poly I:C stimulation in CFS/ME participants. Peripheral MCPs may be present in CFS/ME pathophysiology, however further investigation is required to determine their immunological role.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAssociation of Allergy, Asthma and Immunology of Thailand (AAIAT)
dc.publisher.urihttp://apjai-journal.org/
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto8
dc.relation.ispartofjournalAsian Pacific Journal of Allergy and Immunology
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchClinical sciences not elsewhere classified
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode320299
dc.subject.fieldofresearchcode3204
dc.titleInvestigation of mast cell toll-like receptor 3 in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and Systemic Mastocytosis participants using the novel application of autoMACS magnetic separation and flow cytometry
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.facultyGriffith Health, School of Medical Science
gro.rights.copyright© 2017 Asian Pacific Journal of Allergy and Immunology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorStaines, Donald R.
gro.griffith.authorMarshall-Gradisnik, Sonya M.


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