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dc.contributor.authorBarisam, Maryam
dc.contributor.authorSaidi, Mohammad Said
dc.contributor.authorKashaninejad, Navid
dc.contributor.authorVadivelu, Raja
dc.contributor.authorNam-Trung, Nguyen
dc.date.accessioned2018-03-08T04:28:44Z
dc.date.available2018-03-08T04:28:44Z
dc.date.issued2017
dc.identifier.issn2072-666X
dc.identifier.doi10.3390/mi8120358
dc.identifier.urihttp://hdl.handle.net/10072/370796
dc.description.abstractA microfluidic system provides an excellent platform for cellular studies. Most importantly, a three-dimensional (3D) cell culture model reconstructs more accurately the in vivo microenvironment of tissue. Accordingly, microfluidic 3D cell culture devices could be ideal candidates for in vitro cell culture platforms. In this paper, two types of 3D cellular aggregates, i.e., toroid and spheroid, are numerically studied. The studies are carried out for microfluidic systems containing U-shaped barrier as well as microwell structure. For the first time, we obtain oxygen and glucose concentration distributions inside a toroid aggregate as well as the shear stress on its surface and compare its performance with a spheroid aggregate of the same volume. In particular, we obtain the oxygen concentration distributions in three areas, namely, oxygen-permeable layer, multicellular aggregates and culture medium. Further, glucose concentration distributions in two regions of multicellular aggregates and culture medium are investigated. The results show that the levels of oxygen and glucose in the system containing U-shaped barriers are far more than those in the system containing microwells. Therefore, to achieve high levels of oxygen and nutrients, a system with U-shaped barriers is more suited than the conventional traps, but the choice between toroid and spheroid depends on their volume and orientation. The results indicate that higher oxygen and glucose concentrations can be achieved in spheroid with a small volume as well as in horizontal toroid with a large volume. The vertical toroid has the highest levels of oxygen and glucose concentration while the surface shear stress on its surface is also maximum. These findings can be used as guidelines for designing an optimum 3D microfluidic bioreactor based on the desired levels of oxygen, glucose and shear stress distributions.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofpagefrom358-1
dc.relation.ispartofpageto358-16
dc.relation.ispartofissue12
dc.relation.ispartofjournalMicromachines
dc.relation.ispartofvolume8
dc.subject.fieldofresearchNanotechnology not elsewhere classified
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchcode100799
dc.subject.fieldofresearchcode1007
dc.titleNumerical Simulation of the Behavior of Toroidal and Spheroidal Multicellular Aggregates in Microfluidic Devices with Microwell and U-Shaped Barrier
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorNguyen, Nam-Trung
gro.griffith.authorVadivelu, Raja
gro.griffith.authorKashaninejad, Navid


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