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dc.contributor.authorCampisano, A
dc.contributor.authorSchroeder, C
dc.contributor.authorSchemionek, M
dc.contributor.authorOverhage, J
dc.contributor.authorRehm, BHA
dc.date.accessioned2018-03-13T03:07:42Z
dc.date.available2018-03-13T03:07:42Z
dc.date.issued2006
dc.identifier.issn0099-2240
dc.identifier.doi10.1128/AEM.72.4.3066-3068.2006
dc.identifier.urihttp://hdl.handle.net/10072/371081
dc.description.abstractThe function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region restored the wild-type phenotype. A C-terminal, hexahistidine tag fusion enabled the identification of PslD. LacZ and PhoA translational fusions with PslD indicated that PslD is a secreted protein required for biofilm formation, presumably via its role in exopolysaccharide export.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofpagefrom3066
dc.relation.ispartofpageto3068
dc.relation.ispartofissue4
dc.relation.ispartofjournalApplied and Environmental Microbiology
dc.relation.ispartofvolume72
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchcode060199
dc.titlePslD is a secreted protein required for biofilm formation by Pseudomonas aeruginosa
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.hasfulltextFull Text
gro.griffith.authorRehm, Bernd


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