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dc.contributor.authorRehm, Bernd HA
dc.date.accessioned2018-03-13T03:30:14Z
dc.date.available2018-03-13T03:30:14Z
dc.date.issued2007
dc.identifier.issn1467-3037
dc.identifier.urihttp://hdl.handle.net/10072/371087
dc.description.abstractBiopolyester (PHAs = polyhydroxyalkanoates) composed of hydroxy fatty acids represent a rather complex class of storage polymers synthesized by various eubacteria and archaea and are deposited as water-insoluble cytoplasmic nano-sized inclusions. These spherical shell-core particles are composed of a polyester core surrounded by phospholipids and proteins. The key enzymes of polyester biosynthesis and polyester particle formation are the polyester synthases, which catalyze the enantio-selective polymerization of (R)-hydroxyacyl- CoA thioesters to polyesters. Various metabolic routes have been identified and established in bacteria to provide substrate for polyester synthases. The role of the polyester synthases in morphogenesis and presumably self-assembly of these natural polyester particles will be described. Although not essential for particle formation, non-covalently attached proteins, the so-called phasins, can be found at the particle surface and are considered as structural proteins. A multiple alignment of 88 polyester synthases indicated an identity varying from 8% to 96% with eight strictly conserved amino acid residues. Protein engineering of polyester synthases and phasins was used to functionalize the polyester particle surface. The current knowledge enables the microbial and biocatalytic production of particles with controlled size, polyester core composition and surface functionality, which suggested numerous potential applications of these biocompatible and biodegradable nanostructures particularly in the medical field.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherCaister Academic Press
dc.publisher.urihttps://www.caister.com/cimb/abstracts/v9/41.html
dc.relation.ispartofpagefrom41
dc.relation.ispartofpageto62
dc.relation.ispartofissue1
dc.relation.ispartofjournalCurrent Issues in Molecular Biology
dc.relation.ispartofvolume9
dc.subject.fieldofresearchMedical Microbiology not elsewhere classified
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode110899
dc.subject.fieldofresearchcode1108
dc.titleBiogenesis of microbial polyhydroxyalkanoate granules: A platform technology for the production of tailor-made bioparticles
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorRehm, Bernd


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