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  • The in vitro effects of a single exposure of LASER irradiation on human breast carcinoma and immortalised human mammary epithelial cell lines

    Author(s)
    Powell, Katie
    Laakso, Liisa
    McDonnell, Ann
    Ralph, Stephen
    Griffith University Author(s)
    McDonnell, Ann A.
    Laakso, Liisa
    Ralph, Stephen J.
    Low, Pauline
    Powell, Katie
    Year published
    2008
    Metadata
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    Abstract
    The in vitro effects of a single exposure of LASER irradiation on human breast carcinoma and immortalised human mammary epithelial cell lines. Katie Powell(1&2), Dr Liisa Laakso(2), Dr Ann McDonnell(1), Dr Pauline Low(2), Dr Steve Ralph(2); Griffith University, Brisbane(1) and Gold Coast(2), Australia. PURPOSE: This research examined the cell proliferative response of two breast cancer cell lines and two immortalised human mammary epithelial cell lines in vitro to a range of doses of low level laser therapy (LLLT) at wavelengths of 780 nm, 830 nm and 904 nm. RELEVANCE: LLLT is used in the ...
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    The in vitro effects of a single exposure of LASER irradiation on human breast carcinoma and immortalised human mammary epithelial cell lines. Katie Powell(1&2), Dr Liisa Laakso(2), Dr Ann McDonnell(1), Dr Pauline Low(2), Dr Steve Ralph(2); Griffith University, Brisbane(1) and Gold Coast(2), Australia. PURPOSE: This research examined the cell proliferative response of two breast cancer cell lines and two immortalised human mammary epithelial cell lines in vitro to a range of doses of low level laser therapy (LLLT) at wavelengths of 780 nm, 830 nm and 904 nm. RELEVANCE: LLLT is used in the clinical treatment of post-mastectomy lymphoedema, although specific information on safety is scant and circumstantial. This presentation describes the first steps in gaining disease-specific knowledge upon which to develop guidelines for the safe clinical use of lasers in the management of post-mastectomy lymphoedema. METHODS: Human breast adenocarcinoma (MCF-7), human breast ductal carcinoma (MDA-MB-435S) and immortalised human mammary epithelial (SVCT and Bre80hTERT) cell lines were irradiated with a single exposure of laser at 0.5, 1, 2, 3, 4, 10 and 12 J/cm2 (?=780 nm) and 0.5, 1, 2, 3, 4, 10 and 15 J/cm2 (?=830 nm and 904 nm). Cell proliferation was assessed 24 hours after laser using XTT colorimetric assays. RESULTS: SVCT cell proliferation significantly increased after exposure to doses of 0.5, 1, 2, 3, 4, and 12 J/cm2 of laser at 780 nm and doses of 0.5, 1, 2, 4 and 10 J/cm2 of laser at 904 nm. The MDA-MB-435S and Bre80hTERT cell lines showed negligible effects with one exposure from all three wavelengths of laser and no dose response relationships were noted. The MCF-7 cells demonstrated an increasing dose response relationship with one exposure of 780 nm laser; and there were no dose response effects evident after exposure to 830 nm and 904 nm laser irradiation. CONCLUSIONS: Even though certain doses of laser increased MCF-7 cell proliferation, the amount of laser light penetrating through the skin clinically is considerably attenuated. Further in vivo research of LLLT is required before a definitive conclusion can be made regarding the safety of LLLT for post-mastectomy lymphoedema.
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    Conference Title
    Laser Therapy Vol 17 No 1, March 2008
    Publication URI
    http://hdl.handle.net/10072/37109
    Collection
    • Conference outputs

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