dc.contributor.author | Overhage, Joerg | |
dc.contributor.author | Campisano, Andrea | |
dc.contributor.author | Bains, Manjeet | |
dc.contributor.author | Torfs, Ellen CW | |
dc.contributor.author | Rehm, Bernd HA | |
dc.contributor.author | Hancock, Robert EW | |
dc.date.accessioned | 2018-03-13T03:43:17Z | |
dc.date.available | 2018-03-13T03:43:17Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 0019-9567 | |
dc.identifier.doi | 10.1128/IAI.00318-08 | |
dc.identifier.uri | http://hdl.handle.net/10072/371094 | |
dc.description.abstract | The ability to form biofilms is a critical factor in chronic infections by Pseudomonas aeruginosa and has made this bacterium a model organism with respect to biofilm formation. This study describes a new, previously unrecognized role for the human cationic host defense peptide LL-37. In addition to its key role in modulating the innate immune response and weak antimicrobial activity, LL-37 potently inhibited the formation of bacterial biofilms in vitro. This occurred at the very low and physiologically meaningful concentration of 0.5 μg/ml, far below that required to kill or inhibit growth (MIC = 64 μg/ml). LL-37 also affected existing, pregrown P. aeruginosa biofilms. Similar results were obtained using the bovine neutrophil peptide indolicidin, but no inhibitory effect on biofilm formation was detected using subinhibitory concentrations of the mouse peptide CRAMP, which shares 67% identity with LL-37, polymyxin B, or the bovine bactenecin homolog Bac2A. Using microarrays and follow-up studies, we were able to demonstrate that LL-37 affected biofilm formation by decreasing the attachment of bacterial cells, stimulating twitching motility, and influencing two major quorum sensing systems (Las and Rhl), leading to the downregulation of genes essential for biofilm development. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | American Society for Microbiology | |
dc.relation.ispartofpagefrom | 4176 | |
dc.relation.ispartofpageto | 4182 | |
dc.relation.ispartofissue | 9 | |
dc.relation.ispartofjournal | Infection and Immunity | |
dc.relation.ispartofvolume | 76 | |
dc.subject.fieldofresearch | Biological sciences | |
dc.subject.fieldofresearch | Biochemistry and cell biology not elsewhere classified | |
dc.subject.fieldofresearch | Agricultural, veterinary and food sciences | |
dc.subject.fieldofresearch | Biomedical and clinical sciences | |
dc.subject.fieldofresearchcode | 31 | |
dc.subject.fieldofresearchcode | 310199 | |
dc.subject.fieldofresearchcode | 30 | |
dc.subject.fieldofresearchcode | 32 | |
dc.title | Human host defense peptide LL-37 prevents bacterial biofilm formation | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dc.description.version | Version of Record (VoR) | |
gro.rights.copyright | © 2008 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Rehm, Bernd | |