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  • In vivo monitoring of PHA granule formation using GFP-labeled PHA synthases

    Author(s)
    Peters, V
    Rehm, BHA
    Griffith University Author(s)
    Rehm, Bernd
    Year published
    2005
    Metadata
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    Abstract
    For the first time a functional protein was fused to a PHA synthase resulting in PHA granule formation and display of the respective function at the PHA granule surface. The GFP reporter protein was N-terminally fused to the class I PHA synthase ofCupriavidus necator (PhaC) and the class II PHA synthase ofPseudomonas aeruginosa PAO1 (PhaC1), respectively, while maintaining PHA synthase activity and PHA granule formation. Fluorescence microscopy studies of GFP–PHA synthase attached to emerging PHA granules indicated that emerging PHA granules locate to cell poles and to midcell representing the future cell poles. A rapid ...
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    For the first time a functional protein was fused to a PHA synthase resulting in PHA granule formation and display of the respective function at the PHA granule surface. The GFP reporter protein was N-terminally fused to the class I PHA synthase ofCupriavidus necator (PhaC) and the class II PHA synthase ofPseudomonas aeruginosa PAO1 (PhaC1), respectively, while maintaining PHA synthase activity and PHA granule formation. Fluorescence microscopy studies of GFP–PHA synthase attached to emerging PHA granules indicated that emerging PHA granules locate to cell poles and to midcell representing the future cell poles. A rapid oscillating movement of GFP–PHA synthase foci from pole to pole was observed. In cell division impairedEscherichia coli, PHA granules were localized between nucleoids at regular spacing suggesting that nucleoid occlusion occurred. Accordingly, anucleate regions of theE. coli mukB mutant showed no regular spacing, but PHA granules with twofold increased diameter were formed. First evidence was provided that the cell division and the localization of GFP–PHA synthase foci are in vivo co-located.
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    Journal Title
    FEMS Microbiology Letters
    Volume
    248
    Issue
    1
    DOI
    https://doi.org/10.1016/j.femsle.2005.05.027
    Subject
    Biochemistry and Cell Biology not elsewhere classified
    Biological Sciences
    Agricultural and Veterinary Sciences
    Medical and Health Sciences
    Publication URI
    http://hdl.handle.net/10072/371546
    Collection
    • Journal articles

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