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dc.contributor.authorRehm, BHA
dc.contributor.authorMitsky, TA
dc.contributor.authorSteinbuchel, A
dc.date.accessioned2018-07-10T01:30:31Z
dc.date.available2018-07-10T01:30:31Z
dc.date.issued2001
dc.identifier.issn0099-2240
dc.identifier.doi10.1128/AEM.67.7.3102-3109.2001
dc.identifier.urihttp://hdl.handle.net/10072/372937
dc.description.abstractSince Pseudomonas aeruginosa is capable of biosynthesis of polyhydroxyalkanoic acid (PHA) and rhamnolipids, which contain lipid moieties that are derived from fatty acid biosynthesis, we investigated various fab mutants from P. aeruginosa with respect to biosynthesis of PHAs and rhamnolipids. All isogenicfabA, fabB, fabI, rhlG, and phaG mutants fromP. aeruginosa showed decreased PHA accumulation and rhamnolipid production. In the phaG (encoding transacylase) mutant rhamnolipid production was only slightly decreased. Expression of phaG from Pseudomonas putida and expression of the β-ketoacyl reductase generhlG from P. aeruginosain these mutants indicated that PhaG catalyzes diversion of intermediates of fatty acid de novo biosynthesis towards PHA biosynthesis, whereas RhlG catalyzes diversion towards rhamnolipid biosynthesis. These data suggested that both biosynthesis pathways are competitive. In order to investigate whether PhaG is the only linking enzyme between fatty acid de novo biosynthesis and PHA biosynthesis, we generated five Tn5mutants of P. putida strongly impaired in PHA production from gluconate. All mutants were complemented by thephaG gene from P. putida, indicating that the transacylase-mediated PHA biosynthesis route represents the only metabolic link between fatty acid de novo biosynthesis and PHA biosynthesis in this bacterium. The transacylase-mediated PHA biosynthesis route from gluconate was established in recombinantE. coli, coexpressing the class II PHA synthase genephaC1 together with the phaG gene from P. putida, only when fatty acid de novo biosynthesis was partially inhibited by triclosan. The accumulated PHA contributed to 2 to 3% of cellular dry weight.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofpagefrom3102
dc.relation.ispartofpageto3109
dc.relation.ispartofissue7
dc.relation.ispartofjournalApplied and Environmental Microbiology
dc.relation.ispartofvolume67
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchcode310799
dc.titleRole of Fatty Acid de novo Biosynthesis in Polyhydroxyalkanoic Acid (PHA) and Rhamnolipid Synthesis by Pseudomonads: Establishment of the Transacylase (PhaG)-Mediated Pathway for PHA Biosynthesis in Escherichia coli
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2001 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorRehm, Bernd


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