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dc.contributor.convenorProf Jan Bjordalen_AU
dc.contributor.authorLaakso, Liisaen_US
dc.contributor.authorRenno, Ana Claudiaen_US
dc.contributor.authorMcDonnell, Annen_US
dc.contributor.editorJan Magnus Bjordalen_US
dc.description.abstractBackground and Objective: Our group is investigating the effect of laser phototherapy on a range of cell lines seeded onto novel bioscaffolds as a potential method for assisting in tissue repair and healing. Our aim was to analyse the cell proliferation and growth characteristics of the olfactory neuronal ensheathing cells on the chosen bioscaffolds. Methods: Olfactory lamina propria ensheathing (OLF442) cells were cultured, seeded and grown on two novel scaffolds: (1) fully crystallised bioactive glass-ceramic, and (2) modified spongin collagen matrix. Twenty-four hours after seeding of scaffolds, cells were irradiation with a single exposure of laser (830 nm continuous, 30 mW, 10 J/cm2; Smart Laser Medilase, NSW, Australia). Cell proliferation was calculated with spectrophotometry. Scanning electron micrography was utilised to visualise cell growth, migration patterns, and morphology of cells growing on the three-dimensional scaffolds. Results: Laser irradiation produced a 29% mean reduction in OLF442 cell growth on the glass-ceramic scaffold discs (mean absorbance +/- SD = 0.192+/-0.002) compared to control (non-irradiated) discs (mean absorbance +/- SD = 0.22+/-0.002). There was a 43% mean increase in OLF442 cell proliferation on the modified collagen scaffolds compared to non-irradiated control scaffolds. Conclusion: Both scaffolds were capable of supporting OLF442 cell growth. There was a stimulatory effect of phototherapy on OLF442 cells in collagen derived scaffolds, and inhibitory effect of the same laser attributes on a glass-ceramic scaffold. The results support the potential use of laser for promoting tissue healing using bioscaffolds but the type of scaffold may affect the laser-mediated outcomes.en_US
dc.publisherWorld Association for Laser Therapyen_US
dc.relation.ispartofconferencename8th International Congress of the World Association for Laser Therapyen_US
dc.relation.ispartofconferencetitle8th International Congress of the World Association for Laser Therapy - WALTen_US
dc.relation.ispartoflocationBergen Norwayen_US
dc.subject.fieldofresearchComplementary and Alternative Medicine not elsewhere classifieden_US
dc.titleEffect of 830nm laser phototherapy on olfactory neuronal ensheathing (progenitor-like) cells grown in vitro on bioscaffoldsen_US
dc.typeConference outputen_US
dc.type.descriptionE3 - Conference Publications (Extract Paper)en_US
dc.type.codeE - Conference Publicationsen_US
gro.facultyGriffith Health, School of Allied Health Sciencesen_US
gro.hasfulltextNo Full Text

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    Contains papers delivered by Griffith authors at national and international conferences.

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