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dc.contributor.convenorProf Jan Bjordal
dc.contributor.authorMuniz Renno, Ana Claudia
dc.contributor.authorMcDonnell, Pauline Ann
dc.contributor.authorCrovace, Murilo Camuri
dc.contributor.authorZanotto, Edgar Dutra
dc.contributor.authorLaakso, E-Liisa
dc.contributor.editorJan Magnus Bjordal
dc.date.accessioned2017-05-03T14:11:57Z
dc.date.available2017-05-03T14:11:57Z
dc.date.issued2015
dc.date.modified2011-03-16T07:56:45Z
dc.identifier.issn2280-8000
dc.identifier.urihttp://hdl.handle.net/10072/37297
dc.description.abstractBackground and Objective: Our group is investigating the effect of laser phototherapy on a range of cell lines seeded onto novel bioscaffolds as a potential method for assisting in tissue repair and healing. Our aim was to analyse the cell proliferation and growth characteristics of the olfactory neuronal ensheathing cells on the chosen bioscaffolds. Methods: Olfactory lamina propria ensheathing (OLF442) cells were cultured, seeded and grown on two novel scaffolds: (1) fully crystallised bioactive glass-ceramic, and (2) modified spongin collagen matrix. Twenty-four hours after seeding of scaffolds, cells were irradiation with a single exposure of laser (830 nm continuous, 30 mW, 10 J/cm2; Smart Laser Medilase, NSW, Australia). Cell proliferation was calculated with spectrophotometry. Scanning electron micrography was utilised to visualise cell growth, migration patterns, and morphology of cells growing on the three-dimensional scaffolds. Results: Laser irradiation produced a 29% mean reduction in OLF442 cell growth on the glass-ceramic scaffold discs (mean absorbance +/- SD = 0.192+/-0.002) compared to control (non-irradiated) discs (mean absorbance +/- SD = 0.22+/-0.002). There was a 43% mean increase in OLF442 cell proliferation on the modified collagen scaffolds compared to non-irradiated control scaffolds. Conclusion: Both scaffolds were capable of supporting OLF442 cell growth. There was a stimulatory effect of phototherapy on OLF442 cells in collagen derived scaffolds, and inhibitory effect of the same laser attributes on a glass-ceramic scaffold. The results support the potential use of laser for promoting tissue healing using bioscaffolds but the type of scaffold may affect the laser-mediated outcomes.
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_AU
dc.publisherWorld Association for Laser Therapy
dc.publisher.placeNorway
dc.publisher.urihttp://www.walt.nu/
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofconferencename8th International Congress of the World Association for Laser Therapy
dc.relation.ispartofconferencetitleJOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS
dc.relation.ispartofdatefrom2010-09-25
dc.relation.ispartofdateto2010-09-28
dc.relation.ispartoflocationUnited States
dc.relation.ispartofpagefromE234
dc.relation.ispartofpagetoE240
dc.relation.ispartofissue3
dc.relation.ispartofvolume13
dc.rights.retentionY
dc.subject.fieldofresearchComplementary and Alternative Medicine not elsewhere classified
dc.subject.fieldofresearchcode110499
dc.titleEffect of 830nm laser phototherapy on olfactory neuronal ensheathing (progenitor-like) cells grown in vitro on bioscaffolds
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dc.type.codeE - Conference Publications
gro.facultyGriffith Health, School of Allied Health Sciences
gro.date.issued2010
gro.hasfulltextNo Full Text
gro.griffith.authorMcDonnell, Ann A.
gro.griffith.authorLaakso, Liisa


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    Contains papers delivered by Griffith authors at national and international conferences.

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