Antibody response of rabbits and cystic fibrosis patients to an alginate-specific outer membrane protein of a mucoid strain of Pseudomonas aeruginosa
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The aim of this work was to study the immunogenicity of an outer membrane (OM) protein (AIgE; 54 kDa) which is produced solely by mucoid, i.e. alginate-producing strains of P. aeruginosa. The source of AIgE used for our study was the mucoid strain CF3/M1 originally isolated from sputum of a cystic fibrosis (CF) patient. The purified non-denatured protein served as antigen to raise polyclonal monospecific anti-AIgE antibodies in rabbits and to assay sera from 41 cystic fibrosis (CF) patients for anti-AIgE antibodies. According to clinical protocols the sputa of 22 CF patients were positive for P. aeruginosa , 18 were negative and one case was unknown. Our ELISA studies showed that high titers of anti-AIgE antibodies (IgG) corresponded well with the infection status of the CF patients. None of 23 control sera derived from healthy volunteers contained significant levels of anti-AIgE antibodies. Thus the ELISA should be considered as a sensitive diagnostic tool for the early detection of mucoid P. aeruginosa infections in CF patients. Furthermore, we suggest to include AIgE in a multicomponent experimental vaccine for potential protection of non-colonized CF patients from colonization with mucoid P. aeruginosa.
Microbiology not elsewhere classified