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  • Ligand identification of the adenosine A2A receptor in self-assembled nanodiscs by affinity mass spectrometry

    Author(s)
    Ma, Jun
    Lu, Yan
    Wu, Dong
    Peng, Yao
    Loa-Kum-Cheung, Wendy
    Peng, Chao
    Quinn, Ronald J
    Shui, Wenqing
    Liu, Zhi-Jie
    Griffith University Author(s)
    Quinn, Ronald J.
    Loa-Kum-Cheung, Wendy
    Year published
    2017
    Metadata
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    Abstract
    A nanodisc is designed as a vehicle to contain GPCRs in a native-like phospholipid environment. It maintains GPCRs in a better thermostability and homogeneity state in a detergent-free buffer compared to a detergent micelle environment. Here, we established an affinity mass spectrometry-based approach to assay the interaction between the adenosine A2A receptor in the nanodisc and eight known ligands. The affinity mass spectrometry results are consistent with the previously published data for binding affinities. The combination of nanodisc and affinity MS techniques allows the identification of the GPCR ligands in a relatively ...
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    A nanodisc is designed as a vehicle to contain GPCRs in a native-like phospholipid environment. It maintains GPCRs in a better thermostability and homogeneity state in a detergent-free buffer compared to a detergent micelle environment. Here, we established an affinity mass spectrometry-based approach to assay the interaction between the adenosine A2A receptor in the nanodisc and eight known ligands. The affinity mass spectrometry results are consistent with the previously published data for binding affinities. The combination of nanodisc and affinity MS techniques allows the identification of the GPCR ligands in a relatively stable, unliganded/apo and native state.
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    Journal Title
    Analytical Methods
    Volume
    9
    Issue
    40
    DOI
    https://doi.org/10.1039/c7ay01891f
    Subject
    Chemical Sciences not elsewhere classified
    Analytical Chemistry
    Other Chemical Sciences
    Publication URI
    http://hdl.handle.net/10072/373160
    Collection
    • Journal articles

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