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dc.contributor.authorMa, Jun
dc.contributor.authorLu, Yan
dc.contributor.authorWu, Dong
dc.contributor.authorPeng, Yao
dc.contributor.authorLoa-Kum-Cheung, Wendy
dc.contributor.authorPeng, Chao
dc.contributor.authorQuinn, Ronald J
dc.contributor.authorShui, Wenqing
dc.contributor.authorLiu, Zhi-Jie
dc.date.accessioned2018-04-13T06:18:25Z
dc.date.available2018-04-13T06:18:25Z
dc.date.issued2017
dc.identifier.issn1759-9660
dc.identifier.doi10.1039/c7ay01891f
dc.identifier.urihttp://hdl.handle.net/10072/373160
dc.description.abstractA nanodisc is designed as a vehicle to contain GPCRs in a native-like phospholipid environment. It maintains GPCRs in a better thermostability and homogeneity state in a detergent-free buffer compared to a detergent micelle environment. Here, we established an affinity mass spectrometry-based approach to assay the interaction between the adenosine A2A receptor in the nanodisc and eight known ligands. The affinity mass spectrometry results are consistent with the previously published data for binding affinities. The combination of nanodisc and affinity MS techniques allows the identification of the GPCR ligands in a relatively stable, unliganded/apo and native state.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofpagefrom5851
dc.relation.ispartofpageto5858
dc.relation.ispartofissue40
dc.relation.ispartofjournalAnalytical Methods
dc.relation.ispartofvolume9
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchOther chemical sciences not elsewhere classified
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode349999
dc.titleLigand identification of the adenosine A2A receptor in self-assembled nanodiscs by affinity mass spectrometry
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorQuinn, Ronald J.


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