Microbial degradation of N,N-dimethylformamide by Paracoccus sp. strain DMF-3 from activated sludge
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Author(s)
Zhou, Xu
Jin, Wenbiao
Sun, Cuifang
Gao, Shu-Hong
Chen, Chuan
Wang, Qing
Han, Song-Fang
Tu, Renjie
Latif, Muhammad A
Wang, Qilin
Year published
2018
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N,N-dimethylformamide (DMF) was a typical toxic chemical and existed extensively in industrial wastewater. In this study, a strain of the high-efficient DMF degrading bacteria DMF-3 belonging to Paracoccus sp. was enriched and isolated from activated sludge. The removal rate of DMF by DMF-3 was up to 100% while 1000 mg/L DMF was used as the sole carbon and nitrogen source. Growth kinetics model of DMF-3 was thus established, and the kinetic constants were determined with maximum specific growth rate μmax = 0.22 (h−1), saturation constant Ks = 0.41 (g/L) and inhibition constant Ki = 25.93 (g/L). Based on the analysis of the ...
View more >N,N-dimethylformamide (DMF) was a typical toxic chemical and existed extensively in industrial wastewater. In this study, a strain of the high-efficient DMF degrading bacteria DMF-3 belonging to Paracoccus sp. was enriched and isolated from activated sludge. The removal rate of DMF by DMF-3 was up to 100% while 1000 mg/L DMF was used as the sole carbon and nitrogen source. Growth kinetics model of DMF-3 was thus established, and the kinetic constants were determined with maximum specific growth rate μmax = 0.22 (h−1), saturation constant Ks = 0.41 (g/L) and inhibition constant Ki = 25.93 (g/L). Based on the analysis of the intermediate products, it was found that DMF was firstly converted into dimethylamine and formic acid, and these intermediates were finally degraded to ammonia and carbon dioxide following the typical metabolic pathway of methylotrophs. In addition, to enhance the degrading capacity of DMF-3 in high DMF concentration, ultraviolet mutagenesis process was applied. Compared with the usage of the original strain of DMF-3, the degradation rate was increased by 14.8% with the usage of obtained mutant strain with applied initial DMF concentration 10,000 mg/L.
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View more >N,N-dimethylformamide (DMF) was a typical toxic chemical and existed extensively in industrial wastewater. In this study, a strain of the high-efficient DMF degrading bacteria DMF-3 belonging to Paracoccus sp. was enriched and isolated from activated sludge. The removal rate of DMF by DMF-3 was up to 100% while 1000 mg/L DMF was used as the sole carbon and nitrogen source. Growth kinetics model of DMF-3 was thus established, and the kinetic constants were determined with maximum specific growth rate μmax = 0.22 (h−1), saturation constant Ks = 0.41 (g/L) and inhibition constant Ki = 25.93 (g/L). Based on the analysis of the intermediate products, it was found that DMF was firstly converted into dimethylamine and formic acid, and these intermediates were finally degraded to ammonia and carbon dioxide following the typical metabolic pathway of methylotrophs. In addition, to enhance the degrading capacity of DMF-3 in high DMF concentration, ultraviolet mutagenesis process was applied. Compared with the usage of the original strain of DMF-3, the degradation rate was increased by 14.8% with the usage of obtained mutant strain with applied initial DMF concentration 10,000 mg/L.
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Journal Title
Chemical Engineering Journal
Volume
343
Copyright Statement
© 2018 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
Subject
Chemical engineering
Civil engineering
Environmental engineering
Environmentally sustainable engineering
Global and planetary environmental engineering