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dc.contributor.authorRehm, BHA
dc.contributor.authorSteinbuchel, A
dc.date.accessioned2018-04-20T03:43:53Z
dc.date.available2018-04-20T03:43:53Z
dc.date.issued2001
dc.identifier.issn0175-7598
dc.identifier.doi10.1007/s002530000541
dc.identifier.urihttp://hdl.handle.net/10072/373497
dc.description.abstractThe acyl–acyl carrier protein (ACP) thioesterase cDNA from the plant Umbellularia californica was functionally expressed in various recombinant Escherichia coli strains in order to establish a new metabolic route toward medium-chain-length polyhydroxyalkanoate (PHAMCL) biosynthesis from non-related carbon sources. Coexpression of the PHA synthase genes from Ralstonia eutropha and Pseudomonas aeruginosa, or only the PHA synthase gene from P. aeruginosa, respectively, showed PHAMCL accumulation when the type II PHA synthase from P. aeruginosa was produced. Both wild-type E. coli and various fad mutants were investigated; and only when the β-oxidation pathway was impaired PHAMCL accumulation from gluconate was observed, contributing to about 6% of cellular dry weight. Thus coexpression of type II PHA synthase gene with cDNA encoding the medium-chain acyl-ACP thioesterase from U. californica established a new PHAMCL biosynthesis pathway, connecting fatty acid de novo biosynthesis with fatty acid β-oxidation, using a non-related carbon source.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherSpringer
dc.relation.ispartofpagefrom205
dc.relation.ispartofpageto209
dc.relation.ispartofissue2
dc.relation.ispartofjournalApplied Microbiology and Biotechnology
dc.relation.ispartofvolume55
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchcode060599
dc.titleHeterologous expression of the acyl-acyl carrier protein thioesterase gene from the plant Umbellularia californica mediates polyhydroxyalkanoate biosynthesis in recombinant Escherichia coli
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorRehm, Bernd


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