Show simple item record

dc.contributor.authorRehm, Bernd H.A.
dc.contributor.authorSteinbuchel, Alexander
dc.date.accessioned2018-04-20T03:43:53Z
dc.date.available2018-04-20T03:43:53Z
dc.date.issued2001
dc.identifier.issn0175-7598en_US
dc.identifier.doi10.1007/s002530000541en_US
dc.identifier.urihttp://hdl.handle.net/10072/373497
dc.description.abstractThe acyl–acyl carrier protein (ACP) thioesterase cDNA from the plant Umbellularia californica was functionally expressed in various recombinant Escherichia coli strains in order to establish a new metabolic route toward medium-chain-length polyhydroxyalkanoate (PHAMCL) biosynthesis from non-related carbon sources. Coexpression of the PHA synthase genes from Ralstonia eutropha and Pseudomonas aeruginosa, or only the PHA synthase gene from P. aeruginosa, respectively, showed PHAMCL accumulation when the type II PHA synthase from P. aeruginosa was produced. Both wild-type E. coli and various fad mutants were investigated; and only when the β-oxidation pathway was impaired PHAMCL accumulation from gluconate was observed, contributing to about 6% of cellular dry weight. Thus coexpression of type II PHA synthase gene with cDNA encoding the medium-chain acyl-ACP thioesterase from U. californica established a new PHAMCL biosynthesis pathway, connecting fatty acid de novo biosynthesis with fatty acid β-oxidation, using a non-related carbon source.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.publisherSpringeren_US
dc.relation.ispartofpagefrom205en_US
dc.relation.ispartofpageto209en_US
dc.relation.ispartofissue2en_US
dc.relation.ispartofjournalApplied Microbiology and Biotechnologyen_US
dc.relation.ispartofvolume55en_US
dc.subject.fieldofresearchMicrobiology not elsewhere classifieden_US
dc.subject.fieldofresearchcode060599en_US
dc.titleHeterologous expression of the acyl-acyl carrier protein thioesterase gene from the plant Umbellularia californica mediates polyhydroxyalkanoate biosynthesis in recombinant Escherichia colien_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record