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dc.contributor.authorPettinari, MJ
dc.contributor.authorVazquez, GJ
dc.contributor.authorSilberschmidt, D
dc.contributor.authorRehm, B
dc.contributor.authorSteinbuchel, A
dc.contributor.authorMendez, BS
dc.date.accessioned2018-07-10T01:30:30Z
dc.date.available2018-07-10T01:30:30Z
dc.date.issued2001
dc.identifier.issn0099-2240
dc.identifier.doi10.1128/AEM.67.11.5331-5334.2001
dc.identifier.urihttp://hdl.handle.net/10072/373502
dc.description.abstractGenes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced intoRalstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofpagefrom5331
dc.relation.ispartofpageto5334
dc.relation.ispartofissue11
dc.relation.ispartofjournalApplied and Environmental Microbiology
dc.relation.ispartofvolume67
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchcode060599
dc.titlePoly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2001 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorRehm, Bernd


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