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  • Formation of short chain length/medium chain length polyhydroxyalkanoate copolymers by fatty acid b-oxidation inhibited Ralstonia eutropha

    Author(s)
    Green, PR
    Kemper, J
    Schechtman, L
    Guo, L
    Satkowski, M
    Fiedler, S
    Steinbuchel, A
    Rehm, BHA
    Griffith University Author(s)
    Rehm, Bernd
    Year published
    2002
    Metadata
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    Abstract
    Ralstonia eutropha has been considered as a bacterium, incorporating hydroxyalkanoates of less than six carbons only into polyhydroxyalkanoates (PHAs). Cells of the wild type cultivated with sodium octanoate as the carbon source in the presence of the fatty acid β-oxidation inhibitor sodium acrylate synthesized PHAs composed of the medium chain length hydroxyalkanoates (3HAMCL) 3-hydroxyhexanoate (3HHx) and 3-hydroxyoctanoate (3HO) as well as of 3-hydroxybutyrate and 3-hydroxyproprionate as revealed by gas chromatography, 1H NMR spectroscopy, and mass spectroscopy. The characterization of the polymer as a tetrapolymer was ...
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    Ralstonia eutropha has been considered as a bacterium, incorporating hydroxyalkanoates of less than six carbons only into polyhydroxyalkanoates (PHAs). Cells of the wild type cultivated with sodium octanoate as the carbon source in the presence of the fatty acid β-oxidation inhibitor sodium acrylate synthesized PHAs composed of the medium chain length hydroxyalkanoates (3HAMCL) 3-hydroxyhexanoate (3HHx) and 3-hydroxyoctanoate (3HO) as well as of 3-hydroxybutyrate and 3-hydroxyproprionate as revealed by gas chromatography, 1H NMR spectroscopy, and mass spectroscopy. The characterization of the polymer as a tetrapolymer was confirmed by differential solvent extraction and measurement of melting and glass transition temperature depression in the purified polymer compared to PHB. These data suggested that the R. eutropha PHA synthase is capable of incorporating longer chain substrates than suggested by previous in vitro studies. Furthermore, expression of the class II PHA synthase gene phaC1 from P. aeruginosa in R. eutropha resulted in the accumulation of PHAs consisting of 3HAMCL contributing about 3−5% to cellular dry weight. These PHAs were composed of nearly equal molar fractions of 3HO and 3-hydroxydecanoate (3HD) with traces of 3HHx. These data indicated that 3HAMCL-CoA thioesters were diverted from the fatty acid β-oxidation pathway towards PHA biosynthesis in recombinant R. eutropha.
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    Journal Title
    Biomacromolecules
    Volume
    3
    Issue
    1
    DOI
    https://doi.org/10.1021/bm015620m
    Subject
    Biochemistry and Cell Biology not elsewhere classified
    Chemical Sciences
    Biological Sciences
    Engineering
    Publication URI
    http://hdl.handle.net/10072/373503
    Collection
    • Journal articles

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