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dc.contributor.authorFaghihi, Mohammad Alien_US
dc.contributor.authorKocerha, Janneten_US
dc.contributor.authorModarresi, Farzanehen_US
dc.contributor.authorG. Engström, Pären_US
dc.contributor.authorChalk, Alistairen_US
dc.contributor.authorP. Brothers, Shaunen_US
dc.contributor.authorKoesema, Ericen_US
dc.contributor.authorSt. Laurent III, Georgesen_US
dc.contributor.authorWahlestedt, Claesen_US
dc.date.accessioned2017-04-24T08:44:06Z
dc.date.available2017-04-24T08:44:06Z
dc.date.issued2010en_US
dc.date.modified2014-10-09T00:54:52Z
dc.identifier.issn19326203en_US
dc.identifier.doi10.1371/journal.pone.0013177en_US
dc.identifier.urihttp://hdl.handle.net/10072/37363
dc.description.abstractNatural antisense transcripts represent a class of regulatory RNA molecules, which are characterized by their complementary sequence to another RNA transcript. Extensive sequencing efforts suggest that natural antisense transcripts are prevalent throughout the mammalian genome; however, their biological significance has not been well defined. We performed a loss-of-function RNA interference (RNAi) screen, which targeted 797 evolutionary conserved antisense transcripts, and found evidence for a regulatory role for a number of natural antisense transcripts. Specifically, we found that natural antisense transcripts for CCPG1 and RAPGEF3 may functionally disrupt signaling pathways and corresponding biological phenotypes, such as cell viability, either independently or in parallel with the corresponding sense transcript. Our results show that the large-scale siRNA screen can be applied to evaluate natural antisense transcript modulation of fundamental cellular events.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.format.extent1723086 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom1en_US
dc.relation.ispartofpageto10en_US
dc.relation.ispartofissue10en_US
dc.relation.ispartofjournalPloS Oneen_US
dc.relation.ispartofvolume5en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchGenetics not elsewhere classifieden_US
dc.subject.fieldofresearchcode060499en_US
dc.titleRNAi Screen Indicates Widespread Biological Function for Human Natural Antisense Transcriptsen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright 2010 Faghih et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)en_US
gro.date.issued2010
gro.hasfulltextFull Text


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