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dc.contributor.authorFaghihi, Mohammad Ali
dc.contributor.authorKocerha, Jannet
dc.contributor.authorModarresi, Farzaneh
dc.contributor.authorG. Engström, Pär
dc.contributor.authorChalk, Alistair
dc.contributor.authorP. Brothers, Shaun
dc.contributor.authorKoesema, Eric
dc.contributor.authorSt. Laurent III, Georges
dc.contributor.authorWahlestedt, Claes
dc.date.accessioned2017-05-03T11:48:25Z
dc.date.available2017-05-03T11:48:25Z
dc.date.issued2010
dc.date.modified2014-10-09T00:54:52Z
dc.identifier.issn19326203
dc.identifier.doi10.1371/journal.pone.0013177
dc.identifier.urihttp://hdl.handle.net/10072/37363
dc.description.abstractNatural antisense transcripts represent a class of regulatory RNA molecules, which are characterized by their complementary sequence to another RNA transcript. Extensive sequencing efforts suggest that natural antisense transcripts are prevalent throughout the mammalian genome; however, their biological significance has not been well defined. We performed a loss-of-function RNA interference (RNAi) screen, which targeted 797 evolutionary conserved antisense transcripts, and found evidence for a regulatory role for a number of natural antisense transcripts. Specifically, we found that natural antisense transcripts for CCPG1 and RAPGEF3 may functionally disrupt signaling pathways and corresponding biological phenotypes, such as cell viability, either independently or in parallel with the corresponding sense transcript. Our results show that the large-scale siRNA screen can be applied to evaluate natural antisense transcript modulation of fundamental cellular events.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent1723086 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto10
dc.relation.ispartofissue10
dc.relation.ispartofjournalPloS One
dc.relation.ispartofvolume5
dc.rights.retentionY
dc.subject.fieldofresearchGenetics not elsewhere classified
dc.subject.fieldofresearchcode060499
dc.titleRNAi Screen Indicates Widespread Biological Function for Human Natural Antisense Transcripts
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.rights.copyright© 2010 Faghih et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.date.issued2010
gro.hasfulltextFull Text
gro.griffith.authorChalk, Alistair M.


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