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dc.contributor.authorThaochan, N
dc.contributor.authorDrew, RAI
dc.contributor.authorHughes, JM
dc.contributor.authorVijaysegaran, S
dc.contributor.authorChinajariyawong, A
dc.date.accessioned2017-05-03T12:38:28Z
dc.date.available2017-05-03T12:38:28Z
dc.date.issued2010
dc.date.modified2011-03-17T06:57:03Z
dc.identifier.issn1536-2442
dc.identifier.doi10.1673/031.010.13101
dc.identifier.urihttp://hdl.handle.net/10072/37367
dc.description.abstractBacteria were isolated from the crop and midgut of field collected Bactrocera cacuminata (Hering) and Bactrocera tryoni (Froggatt) (Diptera: Tephritidae). Two methods were used, firstly isolation onto two types of bacteriological culture media (PYEA and TSA) and identification using the API-20E diagnostic kit, and secondly, analysis of samples using the 16S rRNA gene molecular diagnostic method. Using the API-20E method, 10 genera and 17 species of bacteria in the family Enterobacteriaceae were identified from cultures growing on the nutrient agar. The dominant species in both the crop and midgut were Citrobacter freundii, Enterobacter cloacae and Klebsiella oxytoca. Providencia rettgeri, Klebsiella pneumoniae ssp ozaenae and Serratia marcescens were isolated from B. tryoni only. Using the molecular cloning technique that is based on 16S rRNA gene sequences, five bacteria classes were dignosed - Alpha-, Beta-, Gamma- and Delta- Proteobacteria and Firmicutes - including five families, Leuconostocaceae, Enterococcaceae, Acetobacteriaceae, Comamonadaceae and Enterobacteriaceae. The bacteria affiliated with Firmicutes were found mainly in the crop while the Gammaproteobacteria, especially the family Enterobacteriaceae, was dominant in the midgut. This paper presents results from the first known application of molecular cloning techniques to study bacteria within tephritid species and the first record of Firmicutes bacteria in these flies.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent573837 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherUniversity of Wisconsin Library
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto16
dc.relation.ispartofissue131
dc.relation.ispartofjournalJournal of Insect Science
dc.relation.ispartofvolume10
dc.rights.retentionY
dc.subject.fieldofresearchTerrestrial ecology
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchcode310308
dc.subject.fieldofresearchcode3109
dc.titleAlimentary tract bacteria isolated and identified with API-20E and molecular cloning techniques from Australian tropical fruit flies, Bactrocera cacuminata and B. Tryoni
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/3.0/
gro.rights.copyright© 2010 Authors.This is an open access paper. http://creativecommons.org/licenses/by/3.0/ license that permits unrestricted use, provided that the paper is properly attributed.
gro.date.issued2010
gro.hasfulltextFull Text
gro.griffith.authorHughes, Jane M.
gro.griffith.authorDrew, Dick A.


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