Show simple item record

dc.contributor.authorPeart, J
dc.contributor.authorMatherne, GP
dc.contributor.authorCerniway, RJ
dc.contributor.authorHeadrick, JP
dc.date.accessioned2017-05-03T13:32:17Z
dc.date.available2017-05-03T13:32:17Z
dc.date.issued2001
dc.date.modified2007-03-14T02:32:35Z
dc.identifier.issn0008-6363
dc.identifier.urihttp://hdl.handle.net/10072/3752
dc.description.abstractObjectives: To characterize the 'anti-ischemic' effects of adenosine metabolism inhibition in ischemic-reperfused myocardium. Methods: Perfused C57/B16 mouse hearts were subjected to 20 min ischemia 40 min reperfusion in the absence or presence of adenosine deaminase inhibition (50 占erythro-2-(2-hydroxy-3-nonyl)adenine; EHNA) adenosine kinase inhibition (10 占iodotubercidin; IODO), or 10 占adenosine. Hearts overexpressing A1 adenosine receptors (A1ARs) were also studied. Results: EHNA treatment reduced ischemic contracture and post-ischemic diastolic pressure (14Რvs. 20ᱠmmHg), increased recovery of developed pressure (66᳠vs. 53Ქ) and reduced LDH efflux (8.9ᱮ6 vs. 18.0ᱮ7 I.U./g). IODO also improved functional recovery (to 60Ქ) and reduced LDH efflux (5.3ᱮ7 I.U./g), as did treatment with 10 占adenosine. Protection with EHNA was reversed by co-infusion of IODO or 50 占8-?-sulfophenyltheophylline (adenosine receptor antagonist), but unaltered by 20 占inosine+10 孠hypoxanthine. Similarly, effects of iodotubercidin were inhibited by EHNA and 8-?-sulfophenyltheophylline. A1AR overexpression exerted similar effects to EHNA and EHNA or IODO alone enhanced recovery while EHNA+IODO reduced recovery in transgenic hearts. Functional recoveries and xanthine oxidase reactant levels were unrelated in the groups studied. Conclusions: Adenosine deaminase or kinase inhibition protects from ischemia-reperfusion. Cardioprotection via these enzyme inhibitors requires a functioning purine salvage pathway and involves enhanced adenosine receptor activation. Reduced formation of inosine is unimportant in EHNA-mediated protection.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier Science
dc.publisher.placeNetherlands
dc.publisher.urihttps://academic.oup.com/cardiovascres/article/52/1/120/295362
dc.relation.ispartofpagefrom120
dc.relation.ispartofpageto129
dc.relation.ispartofjournalCardiovascular Research
dc.relation.ispartofvolume52
dc.subject.fieldofresearchCardiovascular medicine and haematology
dc.subject.fieldofresearchcode3201
dc.titleCardioprotection with adenosine metabolism inhibitors in ischemic-reperfused mouse heart
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.rights.copyright© 2001 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.
gro.date.issued2001
gro.hasfulltextNo Full Text
gro.griffith.authorHeadrick, John P.
gro.griffith.authorPeart, Jason N.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record