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dc.contributor.authorCatterall, Kylie
dc.contributor.authorRobertson, David
dc.contributor.authorHudson, Sian
dc.contributor.authorTeasdale, Peter R
dc.contributor.authorWelsh, David T
dc.contributor.authorJohn, Richard
dc.date.accessioned2017-05-03T12:38:11Z
dc.date.available2017-05-03T12:38:11Z
dc.date.issued2010
dc.date.modified2011-03-22T07:06:20Z
dc.identifier.issn0039-9140
dc.identifier.doi10.1016/j.talanta.2010.05.046
dc.identifier.urihttp://hdl.handle.net/10072/37531
dc.description.abstractA need for rapid toxicity techniques has seen recent research into developing new microbiological assays and characterising their toxicity responses using a range of substances. A microbiological bioassay that determines changes in ferricyanide-mediated respiration for toxicity measurement (FM-TOX) shows particular promise. The development and optimisation of an improved FM-TOX method, incorporating novel features, is described using Escherichia coli as the biocatalyst. Omission of an exogenous carbon source, used in previously described FM-TOX assays, substantially improves the assay sensitivity. In addition, the development of a two-step procedure (toxicant exposure followed by determination of microbial respiratory activity) was found to enhance the inhibition of E. coli by 3,5-dichlorophenol and four other toxicants, compared to single-step procedures. Other assay parameters, such as the ferricyanide concentration, exposure times and optical density of the biocatalyst were also optimised, sometimes based on practical aspects. Toxicity tests were carried out using the adopted technique on both organic and inorganic toxicants, with classic sigmoid-shaped dose-response curves observed, as well as some non-standard responses. IC50 data is presented for a number of common toxicants. The optimised assay provides a good foundation for further toxicity testing using E. coli, as well as the potential for expanding the technique to utilise other bacteria with complementary toxicity responses, thereby allowing use of the assay in a range of applications.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom751
dc.relation.ispartofpageto757
dc.relation.ispartofissue2
dc.relation.ispartofjournalTalanta
dc.relation.ispartofvolume82
dc.rights.retentionY
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchAnalytical chemistry not elsewhere classified
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode340199
dc.subject.fieldofresearchcode3499
dc.titleA sensitive, rapid ferricyanide-mediated toxicity bioassay developed using Escherichia coli
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, Griffith School of Environment
gro.date.issued2010
gro.hasfulltextNo Full Text
gro.griffith.authorJohn, Richard


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