Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation

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Author(s)
Wright, Mitchell Henry
Adelskov, Joseph
Greene, Anthony Carlson
Griffith University Author(s)
Year published
2017
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Abstract
Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.
Journal Title
Journal of Microbiology & Biology Education
Volume
18
Issue
2
DOI
Copyright Statement
©2017 Author(s). Published by the American Society for Microbiology. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.
Subject
Medical microbiology not elsewhere classified
Curriculum and pedagogy
Specialist studies in education not elsewhere classified
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