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dc.contributor.authorPerera, Manosha
dc.contributor.authorAl-Hebshi, Nezar Noor
dc.contributor.authorPerera, Irosha
dc.contributor.authorIpe, Deepak
dc.contributor.authorUlett, Glen C
dc.contributor.authorSpeicher, David J
dc.contributor.authorChen, Tsute
dc.contributor.authorJohnson, Newell W
dc.date.accessioned2018-05-29T04:57:07Z
dc.date.available2018-05-29T04:57:07Z
dc.date.issued2017
dc.identifier.issn2000-2297
dc.identifier.doi10.1080/20002297.2017.1385369
dc.identifier.urihttp://hdl.handle.net/10072/376108
dc.description.abstractThe aim of this study was to characterize the mycobiome associated with oral squamous-cell carcinoma (OSCC). DNA was extracted from 52 tissue biopsies (cases: 25 OSCC; controls: 27 intra-oral fibro-epithelial polyps [FEP]) and sequenced for the fungal internal transcribed spacer 2 region using Illumina™ 2 x300bp chemistry. Merged reads were classified to species level using a BLASTN-algorithm with UNITE’s named species sequences as reference. Downstream analyses were performed using QIIME™ and linear discriminant analysis effect size. A total of 364 species representing 160 genera and two phyla (Ascomycota and Basidiomycota) were identified, with Candida and Malassezia making up 48% and 11% of the average mycobiome, respectively. However, only five species and four genera were detected in ≥50% of the samples. The species richness and diversity were significantly lower in OSCC. Genera Candida, Hannaella, and Gibberella were overrepresented in OSCC; Alternaria and Trametes were more abundant in FEP. Species-wise, Candida albicans, Candida etchellsii, and a Hannaella luteola–like species were enriched in OSCC, while a Hanseniaspora uvarum–like species, Malassezia restricta, and Aspergillus tamarii were the most significantly abundant in FEP. In conclusion, a dysbiotic mycobiome dominated by C. albicans was found in association with OSCC, a finding worth further investigation.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherTaylor & Francis
dc.relation.ispartofpagefrom1385369-1
dc.relation.ispartofpageto1385369-9
dc.relation.ispartofissue1
dc.relation.ispartofjournalJournal of Oral Microbiology
dc.relation.ispartofvolume9
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchMedical microbiology not elsewhere classified
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode320799
dc.subject.fieldofresearchcode3107
dc.titleA dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.facultyGriffith Health, School of Dentistry and Oral Health
gro.rights.copyright© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorJohnson, Newell W.
gro.griffith.authorUlett, Glen C.


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