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dc.contributor.authorMamoori, Afraa
dc.contributor.authorWahab, Riajul
dc.contributor.authorIslam, Farhadul
dc.contributor.authorLee, Katherine
dc.contributor.authorVider, Jelena
dc.contributor.authorLu, Cu-Tai
dc.contributor.authorGopalan, Vinod
dc.contributor.authorLam, Alfred King-yin
dc.date.accessioned2019-07-04T12:38:15Z
dc.date.available2019-07-04T12:38:15Z
dc.date.issued2018
dc.identifier.issn0046-8177
dc.identifier.doi10.1016/j.humpath.2017.10.024
dc.identifier.urihttp://hdl.handle.net/10072/376123
dc.description.abstractThis study was to investigate the expression pattern, mechanisms and clinicopathological implications of miR-193a-3p in colorectal cancer. Fresh-frozen tissues from 70 matched colorectal adenocarciomas and the adjacent non-neoplastic mucosae were prospectively collected. Two colorectal cancer cell lines (SW480 and SW48) and a non-neoplastic colon cell line (FHC) were also used. The expression levels of miR193a-3p in the cells and tissues were measured by quantitative real-time polymerase chain reaction. The expression of KRAS protein as a predicted downstream target for miR-193a was studied by immunohistochemistry. Restoration of the miR-193a level in the cell lines by permanent transfection was achieved and multiple functional and immunological assays were performed to analyze the functions of miR-193a in vitro. Down-regulation of miR-193a-3p was noted in 70% of the colorectal cancer tissues when compared to non-neoplastic colorectal tissues. In addition, down-regulation of miR-193a was significantly correlated with carcinoma of early stages (P < .05). Significant inverse correlation between miR-193a-3p and its target KRAS protein was determined (P < .05). Overexpression of miR-193a in colon cancer cells resulted in reduced cell proliferation, increased apoptosis, induced significant changes in cell cycle events and decreased the expression of epithelial-mesenchymal transition marker TWIST. This study confirms the tumor suppressor roles of miR-193a-3p, its downstream target affinity to KRAS and clinical significance in patients with colorectal adenocarcinoma.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited States
dc.relation.ispartofpagefrom145
dc.relation.ispartofpageto156
dc.relation.ispartofjournalHuman Pathology
dc.relation.ispartofvolume71
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchClinical sciences not elsewhere classified
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode320299
dc.titleClinical and biological significance of miR-193a-3p targeted KRAS in colorectal cancer pathogenesis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.description.versionAccepted Manuscript (AM)
gro.facultyAn Unassigned Group, An Unassigned Department
gro.rights.copyright© 2018 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLam, Alfred K.
gro.griffith.authorGopalan, Vinod
gro.griffith.authorVider, Jelena
gro.griffith.authorIslam, Farhad
gro.griffith.authorMamoori, Afraa Mahdi Jawad MJ.
gro.griffith.authorWahab, S. M. Riajul MR.
gro.griffith.authorLee, Katherine K.


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