Identification of Locally Found Bacteria for Potential Use in Ground Improvement Works by Microbially Induced Calcite Precipitation (MICP) Technique
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Recent technology known as Microbially Induced Calcite Precipitation (MICP) has been an important breakthrough in the field of geotechnical engineering. It is a viable technique of ground improvement that incorporates the use of bacteria that act as a nucleation site and further initiates the formation of calcite precipitation. Over the past decade, the development of this technology has increased from small-scale laboratory work to large experiment work as well as the execution of a novel field test conducted in 2011. Most researchers have been using one common bacterium known as Sporosarcina Pasteurii which was also known as Bacillus Pasteurii. It is chosen due to its high positive urease activity that degrades urea and through hydrolysis of urea, the formation of CaCO3 can be realised through the mixture of carbonate ion (CO3 2- ) and the calcium ion (Ca2+) from supplied calcium chloride. This paper describes the isolation and characterization of urease producing bacteria within the vicinity of Kuching, Sarawak. The sampling location of the bacteria is located at the Kuching Wastewater Treatment Plant and was further analysed and identified in the Microbiology Lab of Swinburne University of Technology, Sarawak Campus. Results from Microbact TestTM Kit and Gram Staining Test identified the bacteria as Ps Stutzeri (97.04%), F. Odoratum (66.31%), and Actinobacillus sp. (89.76%). The precipitation of calcite produced through the process of MICP of these locally found bacteria were able to be achieved.
SDPS 2014 Malaysia: Smart Innovative Societies [19th International Conference on Transformative Research in Science and Engineering, Business and Social Innovation]
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