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dc.contributor.authorOmoregie, Armstrong Ighodalo
dc.contributor.authorSenian, Nurnajwani
dc.contributor.authorLi, Phua Ye
dc.contributor.authorHei, Ngu Lock
dc.contributor.authorLeong, Dominic Ong Ek
dc.contributor.authorGinjom, Irine Runnie Henry
dc.contributor.authorNissom, Peter Morin
dc.date.accessioned2018-06-11T03:37:10Z
dc.date.available2018-06-11T03:37:10Z
dc.date.issued2016
dc.identifier.issn1823-8262
dc.identifier.urihttp://hdl.handle.net/10072/376571
dc.description.abstractAims: Microbial induced calcite precipitation (MICP) is a natural occurring biological process that employs the usage of ureolytic bacteria for a wide range of applications such as improving the mechanical properties of soils. The aim of this study was to isolate and identify local urease-producing bacteria from the limestone caves of Sarawak and characterise their specific urease activities. Methodology and results: Enrichment culture technique was used to isolate urease-producing bacteria. These local isolates were identified using phenotypic and molecular characterisation. Conductivity method and biomass (OD600) measurements were conducted to analyze and determine the specific urease activities of the local isolates. 16S rRNA gene sequence analysis identified the bacterial isolates as Sporosarcina pasteurii, Sporosarcina luteola and Bacillus lentus. Conclusion: significance and impact of study: This is the first study reporting the isolation and identification of urease-producing bacteria from Fairy and Wind Caves situated in Bau, Sarawak, Malaysia. The findings in this study suggest the bacterial isolates are capable of inducing calcite precipitation and serve as alternative microbial ureolytic agents.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMalaysian Society for Microbiology
dc.publisher.urihttp://mjm.usm.my/index.php?r=cms/entry/view&id=83&slug=archive
dc.relation.ispartofpagefrom463
dc.relation.ispartofpageto470
dc.relation.ispartofissue6
dc.relation.ispartofjournalMalaysian Journal of Microbiology
dc.relation.ispartofvolume12
dc.subject.fieldofresearchOrganic geochemistry
dc.subject.fieldofresearchEcosystem function
dc.subject.fieldofresearchMicrobial ecology
dc.subject.fieldofresearchcode370304
dc.subject.fieldofresearchcode410203
dc.subject.fieldofresearchcode310703
dc.titleUreolytic bacteria isolated from Sarawak limestone caves show high urease enzyme activity comparable to that of Sporosarcina pasteurii (DSM33)
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by-nc/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2016. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorOng, Dominic E.L.


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