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dc.contributor.authorBulaeva, Elizabeth
dc.contributor.authorLanctot, Chantal
dc.contributor.authorReynolds, Leslie
dc.contributor.authorTrudeau, Vance L
dc.contributor.authorNavarro-Martin, Laia
dc.date.accessioned2018-09-27T02:52:43Z
dc.date.available2018-09-27T02:52:43Z
dc.date.issued2015
dc.identifier.issn0016-6480
dc.identifier.doi10.1016/j.ygcen.2015.01.012
dc.identifier.urihttp://hdl.handle.net/10072/378570
dc.description.abstractNumerous endocrine disrupting chemicals can affect the growth and development of amphibians. We investigated the effects of a targeted disruption of the endocrine axes modulating development and somatic growth. Wood frog (Lithobates sylvaticus) tadpoles were exposed for 2 weeks (from developmental Gosner stage (Gs) 25 to Gs30) to sodium perchlorate (SP, thyroid inhibitor, 14 mg/L), estradiol (E2, known to alter growth and development, 200 nM) and a reduced feeding regime (RF, to affect growth and development in a chemically-independent manner). All treatments experienced developmental delay, and animals exposed to SP or subjected to RF respectively reached metamorphic climax (Gs42) approximately 11(±3) and 17(±3) days later than controls. At Gs42, only SP-treated animals showed increased weight and snout-vent length (P < 0.05) relative to controls. Tadpoles treated with SP had 10-times higher levels of liver igf1 mRNA after 4 days of exposure (Gs28) compared to controls. Tadpoles in the RF treatment expressed 6-times lower levels of liver igf1 mRNA and 2-times higher liver igf1r mRNA (P < 0.05) at Gs30. Tadpoles treated with E2 exhibited similar developmental and growth patterns as controls, but had increased liver igf1 mRNA levels at Gs28, and tail igf1r at Gs42. Effects on tail trβ mRNA levels were detected in SP-treated tadpoles at Gs42, 40 days post-exposure, suggesting that the chemical inhibition of thyroid hormone production early in development can have long-lasting effects. The growth effects observed in the SP-exposed animals suggest a relationship between TH-dependent development and somatic growth in L. sylvaticus tadpoles.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom33
dc.relation.ispartofpageto43
dc.relation.ispartofjournalGeneral and Comparative Endocrinology
dc.relation.ispartofvolume222
dc.subject.fieldofresearchBioavailability and ecotoxicology
dc.subject.fieldofresearchcode410201
dc.titleSodium perchlorate disrupts development and affects metamorphosis- and growth-related gene expression in tadpoles of the wood frog (Lithobates sylvaticus)
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2015 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLanctot, Chantal


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