Show simple item record

dc.contributor.authorNavarro-Martin, Laia
dc.contributor.authorLanctot, Chantal
dc.contributor.authorEdge, Christopher
dc.contributor.authorHoulahan, Jeff
dc.contributor.authorTrudeau, Vance L
dc.date.accessioned2018-07-17T01:52:01Z
dc.date.available2018-07-17T01:52:01Z
dc.date.issued2012
dc.identifier.issn0008-4301
dc.identifier.doi10.1139/z2012-074
dc.identifier.urihttp://hdl.handle.net/10072/378693
dc.description.abstractNumerous studies using laboratory-reared tadpoles have shown the importance of thyroid hormones (TH), thyroid receptors (TR), and deiodinase (Dio) enzymes during anuran metamorphosis. Our study focuses on the analysis of thyroid-related genes in tadpoles of wild Wood Frogs (Lithobates sylvaticus (LeConte, 1825); also known as Rana sylvatica (Cope, 1889)) during metamorphosis. Results showed that, in concordance with laboratory-reared studies, thyroid receptor beta (trb) gene expression profiles presented the most marked changes. At climax and compared with premetamorphic stages, brains, tails, and gonad–mesonephros complex (GMC) tissues increased trb expression levels 5-, 21-, and 41-fold, respectively (p < 0.05). In addition, gene expression levels of brain deiodinase type II and III showed opposite trends, where 3-fold decrease and 10-fold increase were, respectively, found. This finding supports the idea that thyroid hormone, as it has been demonstrated in laboratory-reared tadpoles, is also involved in natural metamorphosis in wild tadpoles. Interestingly, and contrary to our predictions, we observed that whole brain corticotropin-releasing factor (crf) and crf receptor 1 (crfr1) gene expression levels significantly decrease through metamorphosis in wild L. sylvaticus tadpoles. Further analyses are required to determine if a role of TH in the timing of anuran gonadal development exists, as well as the importance of cell-specific and tissue-specific expression of crf and crfr1 to metamorphosis.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherCanadian Science Publishing
dc.relation.ispartofpagefrom1059
dc.relation.ispartofpageto1071
dc.relation.ispartofissue9
dc.relation.ispartofjournalCanadian Journal of Zoology
dc.relation.ispartofvolume90
dc.subject.fieldofresearchDevelopmental Genetics (incl. Sex Determination)
dc.subject.fieldofresearchEcology
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchcode060403
dc.subject.fieldofresearchcode0602
dc.subject.fieldofresearchcode0608
dc.titleExpression profiles of metamorphosis-related genes during natural transformations in tadpoles of wild Wood Frogs (Lithobates sylvaticus)
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorLanctot, Chantal


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record