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dc.contributor.authorFernandez-Becerra, Carmenen_US
dc.contributor.authorSanz, Sergien_US
dc.contributor.authorBrucet, Marinaen_US
dc.contributor.authorStanisic, Danielleen_US
dc.contributor.authorAlves, Fabianaen_US
dc.contributor.authorCamargo, Erneyen_US
dc.contributor.authorAlonso, Pedroen_US
dc.contributor.authorMueller, Ivoen_US
dc.contributor.authordel Portillo, Hernandoen_US
dc.date.accessioned2017-04-24T13:39:13Z
dc.date.available2017-04-24T13:39:13Z
dc.date.issued2010en_US
dc.date.modified2011-04-20T07:46:02Z
dc.identifier.issn1475-2875en_US
dc.identifier.doi10.1186/1475-2875-9-29en_AU
dc.identifier.urihttp://hdl.handle.net/10072/37992
dc.description.abstractBackground Progress towards the development of a malaria vaccine against Plasmodium vivax, the most widely distributed human malaria parasite, will require a better understanding of the immune responses that confer clinical protection to patients in regions where malaria is endemic. Methods Glutathione S-transferase (GST) and GST-fusion proteins representing the N- terminus of the merozoite surface protein 1 of P. vivax, PvMSP1-N, and the C-terminus, PvMSP1-C, were covalently coupled to BioPlex carboxylated beads. Recombinant proteins and coupled beads were used, respectively, in ELISA and Bioplex assays using immune sera of P. vivax patients from Brazil and PNG to determine IgG and subclass responses. Concordances between the two methods in the seropositivity responses were evaluated using the Kappa statistic and the Spearman's rank correlation. Results The results using this methodology were compared with the classical microtitre enzyme-linked immnosorbent assay (ELISA), showing that the assay was sensitive, reproducible and had good concordance with ELISA; yet, further research into different statistical analyses seems desirable before claiming conclusive results exclusively based on multiplex assays. As expected, results demonstrated that PvMSP1 was immunogenic in natural infections of patients from different endemic regions of Brazil and Papua New Guinea (PNG), and that age correlated only with antibodies against the C-terminus part of the molecule. Furthermore, the IgG subclass profiles were different in these endemic regions having IgG3 predominantly recognizing PvMSP1 in Brazil and IgG1 predominantly recognizing PvMSP1 in PNG. Conclusions This study validates the use of the multiplex assay to measure naturally-acquired IgG antibodies against the merozoite surface protein 1 of P. vivax.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.format.extent863346 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherBioMed Centralen_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom1en_US
dc.relation.ispartofpageto8en_US
dc.relation.ispartofissue1en_US
dc.relation.ispartofjournalMalaria Journalen_US
dc.relation.ispartofvolume9en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchHumoural Immunology and Immunochemistryen_US
dc.subject.fieldofresearchcode110705en_US
dc.titleNaturally-acquired humoral immune responses against the N- and C- termini of the Plasmodium vivax MSP1 protein in endemic regions of Brazil and Papua New Guinea using a multiplex assayen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright 2010 Fernandez-Becerra et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_AU
gro.date.issued2010
gro.hasfulltextFull Text


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