Expression of GAEC1 mRNA and protein and its association with clinical and pathological parameters of patients with colorectal adenocarcinoma

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Author(s)
Wahab, Riajul
Gopalan, Vinod
Islam, Farhadul
Mamoori, Afraa
Lee, Katherine Ting-Wei
Lu, Cu-Tai
Lam, Alfred King-Yin
Year published
2018
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Aim: GAEC1 (Gene amplified in esophageal cancer 1) is an oncogene with key regulatory roles in the pathogenesisof oesophageal and colorectal carcinomas. The aim of this study was to investigate expression pro files andclinicopathological significance of GAEC1 mRNA and protein in patients with colorectal carcinomas.Method: Matched cancer and non-cancer fresh frozen tissues were prospectively collected from 80 patients di-agnosed with colorectal adenocarcinoma (39 men and 41 women). The tissues were sectioned for RNA ex-traction and cDNA conversion and quantified by a real-time polymerase chain reaction. GAEC1 protein ex-pression ...
View more >Aim: GAEC1 (Gene amplified in esophageal cancer 1) is an oncogene with key regulatory roles in the pathogenesisof oesophageal and colorectal carcinomas. The aim of this study was to investigate expression pro files andclinicopathological significance of GAEC1 mRNA and protein in patients with colorectal carcinomas.Method: Matched cancer and non-cancer fresh frozen tissues were prospectively collected from 80 patients di-agnosed with colorectal adenocarcinoma (39 men and 41 women). The tissues were sectioned for RNA ex-traction and cDNA conversion and quantified by a real-time polymerase chain reaction. GAEC1 protein ex-pression was analysed by immunohistochemistry using a custom made GAEC1 antibody.Result: GAEC1 mRNA was upregulated in majority (52%, n = 42/80) of the colorectal carcinomas when com-pared to the matched non-neoplastic tissues. High expression of GAEC1 mRNA as correlated with patients ofyounger age (p = 0.008), with lower grade carcinoma (p = 0.028), presence of synchronous adenocarcinomas(p = 0.034) and without any associated adenomas (p = 0.047). In addition, patients with high GAEC1 mRNAoverexpression had a shorter survival time. Furthermore, high GAEC1 protein expression was noted amongpatients having perforated colorectal carcinoma (p = 0.04).Conclusion: The high expression of GAEC1 mRNA/protein as well as its correlation with multiple clin-icopathological characteristics in patients with colorectal carcinoma strongly suggests that GAEC1 is a keyregulator in the initiation of colorectal carcinogenesis.
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View more >Aim: GAEC1 (Gene amplified in esophageal cancer 1) is an oncogene with key regulatory roles in the pathogenesisof oesophageal and colorectal carcinomas. The aim of this study was to investigate expression pro files andclinicopathological significance of GAEC1 mRNA and protein in patients with colorectal carcinomas.Method: Matched cancer and non-cancer fresh frozen tissues were prospectively collected from 80 patients di-agnosed with colorectal adenocarcinoma (39 men and 41 women). The tissues were sectioned for RNA ex-traction and cDNA conversion and quantified by a real-time polymerase chain reaction. GAEC1 protein ex-pression was analysed by immunohistochemistry using a custom made GAEC1 antibody.Result: GAEC1 mRNA was upregulated in majority (52%, n = 42/80) of the colorectal carcinomas when com-pared to the matched non-neoplastic tissues. High expression of GAEC1 mRNA as correlated with patients ofyounger age (p = 0.008), with lower grade carcinoma (p = 0.028), presence of synchronous adenocarcinomas(p = 0.034) and without any associated adenomas (p = 0.047). In addition, patients with high GAEC1 mRNAoverexpression had a shorter survival time. Furthermore, high GAEC1 protein expression was noted amongpatients having perforated colorectal carcinoma (p = 0.04).Conclusion: The high expression of GAEC1 mRNA/protein as well as its correlation with multiple clin-icopathological characteristics in patients with colorectal carcinoma strongly suggests that GAEC1 is a keyregulator in the initiation of colorectal carcinogenesis.
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Journal Title
Experimental and Molecular Pathology
Volume
104
Issue
1
Copyright Statement
© 2018 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
Subject
Clinical sciences
Clinical sciences not elsewhere classified