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dc.contributor.authorNeale, Peta A
dc.contributor.authorBrack, Werner
dc.contributor.authorAit-Aissa, Selim
dc.contributor.authorBusch, Wibke
dc.contributor.authorHollender, Juliane
dc.contributor.authorKrauss, Martin
dc.contributor.authorMaillot-Marechal, Emmanuelle
dc.contributor.authorMunz, Nicole A
dc.contributor.authorSchlichting, Rita
dc.contributor.authorSchulze, Tobias
dc.contributor.authorVogler, Bernadette
dc.contributor.authorEscher, Beate I
dc.date.accessioned2019-06-19T13:08:12Z
dc.date.available2019-06-19T13:08:12Z
dc.date.issued2018
dc.identifier.issn2050-7887
dc.identifier.doi10.1039/c7em00555e
dc.identifier.urihttp://hdl.handle.net/10072/380170
dc.description.abstractIn vitro bioassays are increasingly used for water quality monitoring. Surface water samples often need to be enriched to observe an effect and solid-phase extraction (SPE) is commonly applied for this purpose. The applied methods are typically optimised for the recovery of target chemicals and not for effect recovery for bioassays. A review of the few studies that have evaluated SPE recovery for bioassays showed a lack of experimentally determined recoveries. Therefore, we systematically measured effect recovery of a mixture of 579 organic chemicals covering a wide range of physicochemical properties that were spiked into a pristine water sample and extracted using large volume solid-phase extraction (LVSPE). Assays indicative of activation of xenobiotic metabolism, hormone receptor-mediated effects and adaptive stress responses were applied, with non-specific effects determined through cytotoxicity measurements. Overall, effect recovery was found to be similar to chemical recovery for the majority of bioassays and LVSPE blanks had no effect. Multi-layer SPE exhibited greater recovery of spiked chemicals compared to LVSPE, but the blanks triggered cytotoxicity at high enrichment. Chemical recovery data together with single chemical effect data were used to retrospectively estimate with reverse recovery modelling that there was typically less than 30% effect loss expected due to reduced SPE recovery in published surface water monitoring studies. The combination of targeted experiments and mixture modelling clearly shows the utility of SPE as a sample preparation method for surface water samples, but also emphasizes the need for adequate controls when extraction methods are adapted from chemical analysis workflows.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherRoyal Society of Chemistry
dc.publisher.placeUnited Kingdom
dc.relation.ispartofpagefrom493
dc.relation.ispartofpageto504
dc.relation.ispartofissue3
dc.relation.ispartofjournalEnvironmental Science: Processes and Impacts
dc.relation.ispartofvolume20
dc.relation.urihttp://purl.org/au-research/grants/NHMRC/APP1074775
dc.relation.grantIDAPP1074775
dc.relation.fundersNHMRC
dc.subject.fieldofresearchEnvironmental Sciences not elsewhere classified
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchEnvironmental Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode059999
dc.subject.fieldofresearchcode03
dc.subject.fieldofresearchcode05
dc.subject.fieldofresearchcode11
dc.titleSolid-phase extraction as sample preparation of water samples for cell-based and other in vitro bioassays
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionPost-print
gro.facultyGriffith Sciences, School of Environment and Science
gro.rights.copyright© 2018 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorEscher, Beate
gro.griffith.authorNeale, Peta A.


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