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dc.contributor.authorChim, Wilson
dc.contributor.authorSedighi, Abootaleb
dc.contributor.authorBrown, Christopher L
dc.contributor.authorPantophlet, Ralph
dc.contributor.authorLi, Paul CH
dc.date.accessioned2019-05-29T13:04:30Z
dc.date.available2019-05-29T13:04:30Z
dc.date.issued2018
dc.identifier.issn0008-4042
dc.identifier.doi10.1139/cjc-2017-0319
dc.identifier.urihttp://hdl.handle.net/10072/380371
dc.description.abstractHerein, we report that peptide nucleic acid sequences (PNAs) have been used as the probe species for detection of RNA and that a microfluidic microarray (MMA) chip is used as the platform for detection of hybridizations between immobilized PNA probes and RNA targets. The RNA targets used are derived from influenza A sequences. This paper discusses the optimization of two probe technologies used for RNA detection and investigates how the composition of the probe buffer and the content of the hybridization solution can influence the overall results. Our data show that the PNA probe is a better choice than the DNA probe when there is low salt in the probe buffer composition. Furthermore, we show that the absence of salt (NaCl) in the hybridization buffer does not hinder the detection of RNA sequences. The results provide evidence that PNA probes are superior to DNA probes in term of sensitivity and adaptability, as PNA immobilization and PNA–RNA hybridization are less affected by salt content in the reaction buffers unlike DNA probes.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherNRC Research Press
dc.publisher.placeCanada
dc.relation.ispartofpagefrom241
dc.relation.ispartofpageto247
dc.relation.ispartofissue2
dc.relation.ispartofjournalCanadian Journal of Chemistry
dc.relation.ispartofvolume96
dc.subject.fieldofresearchChemical Sciences not elsewhere classified
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchcode039999
dc.subject.fieldofresearchcode03
dc.titleEffect of buffer composition on PNA-RNA hybridization studied in the microfluidic microarray chip
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionPost-print
gro.rights.copyright© The Author(s) 2018. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorBrown, Chris L.


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