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dc.contributor.authorChim, Wilsonen_US
dc.contributor.authorSedighi, Abootaleben_US
dc.contributor.authorBrown, Christopheren_US
dc.contributor.authorPantophlet, Ralphen_US
dc.contributor.authorLi, Paulen_US
dc.date.accessioned2019-05-29T13:04:30Z
dc.date.available2019-05-29T13:04:30Z
dc.date.issued2018en_US
dc.identifier.issn0008-4042en_US
dc.identifier.doi10.1139/cjc-2017-0319en_US
dc.identifier.urihttp://hdl.handle.net/10072/380371
dc.description.abstractHerein, we report that peptide nucleic acid sequences (PNAs) have been used as the probe species for detection of RNA and that a microfluidic microarray (MMA) chip is used as the platform for detection of hybridizations between immobilized PNA probes and RNA targets. The RNA targets used are derived from influenza A sequences. This paper discusses the optimization of two probe technologies used for RNA detection and investigates how the composition of the probe buffer and the content of the hybridization solution can influence the overall results. Our data show that the PNA probe is a better choice than the DNA probe when there is low salt in the probe buffer composition. Furthermore, we show that the absence of salt (NaCl) in the hybridization buffer does not hinder the detection of RNA sequences. The results provide evidence that PNA probes are superior to DNA probes in term of sensitivity and adaptability, as PNA immobilization and PNA–RNA hybridization are less affected by salt content in the reaction buffers unlike DNA probes.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.publisherNRC Research Pressen_US
dc.publisher.placeCanadaen_US
dc.relation.ispartofpagefrom241en_US
dc.relation.ispartofpageto247en_US
dc.relation.ispartofissue2en_US
dc.relation.ispartofjournalCanadian Journal of Chemistryen_US
dc.relation.ispartofvolume96en_US
dc.subject.fieldofresearchChemical Sciences not elsewhere classifieden_US
dc.subject.fieldofresearchChemical Sciencesen_US
dc.subject.fieldofresearchcode039999en_US
dc.subject.fieldofresearchcode03en_US
dc.titleEffect of buffer composition on PNA-RNA hybridization studied in the microfluidic microarray chipen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dc.type.codeC - Journal Articlesen_US
dc.description.versionPost-printen_US
gro.rights.copyright© The Author(s) 2018. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.en_US
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