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dc.contributor.authorTello Velasquez, Johanaen_US
dc.contributor.authorSt John, Jamesen_US
dc.contributor.authorNazareth, Lynnmariaen_US
dc.contributor.authorEkberg, Jennyen_US
dc.date.accessioned2019-05-29T12:37:40Z
dc.date.available2019-05-29T12:37:40Z
dc.date.issued2018en_US
dc.identifier.issn1044-7431en_US
dc.identifier.doi10.1016/j.mcn.2018.01.001en_US
dc.identifier.urihttp://hdl.handle.net/10072/380783
dc.description.abstractLamellipodia in Schwann cells (SCs) are crucial for myelination, but their other biological functions remain largely uncharacterised. Two types of lamellipodia exist in SCs: axial lamellipodia at the outermost edge of the cell processes, and radial lamellipodia appearing peripherally along the entire cell. We have previously shown that radial lamellipodia on olfactory glia (olfactory ensheathing cells; OECs) promote cell-cell adhesion, contact-mediated migration and phagocytosis. Here we have investigated whether lamellipodia in SCs have similar roles. Using live-cell imaging, we show that the radial lamellipodia in SCs are highly motile, appear at multiple cellular sites and rapidly move in a wave-like manner. We found that axial and radial lamellipodia had strikingly different roles and are regulated by different intracellular pathways. Axial lamellipodia initiated interactions with other SCs and with neurons by contacting radial lamellipodia on SCs, and budding neurites/axons. Most SC-SC interactions resulted in repulsion, and, lamellipodial activity (unlike in OECs) did not promote contact-mediated migration. We show that lamellipodia are crucial for SC-mediated phagocytosis of both axonal debris and bacteria, and demonstrated that inhibition of lamellipodial activity by blocking the Rho/Rac pathways also inhibits phagocytosis. We also show that heregulin, which induces SC differentiation and maturation, alters lamellipodial behaviour but does not affect phagocytic activity. Overall, the results show that SC lamellipodia are important for cell interactions and phagocytosis.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.publisherElsevieren_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofpagefrom189en_US
dc.relation.ispartofpageto200en_US
dc.relation.ispartofjournalMolecular and Cellular Neuroscienceen_US
dc.relation.ispartofvolume88en_US
dc.subject.fieldofresearchNeurosciences not elsewhere classifieden_US
dc.subject.fieldofresearchNeurosciencesen_US
dc.subject.fieldofresearchCognitive Sciencesen_US
dc.subject.fieldofresearchcode110999en_US
dc.subject.fieldofresearchcode1109en_US
dc.subject.fieldofresearchcode1702en_US
dc.titleSchwann cell lamellipodia regulate cell-cell interactions and phagocytosisen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dc.type.codeC - Journal Articlesen_US
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.description.versionPost-printen_US
gro.facultyGriffith Sciences, Griffith Institute for Drug Discoveryen_US
gro.rights.copyright© 2018 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.en_US
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