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dc.contributor.authorVelasquez, Johana Tello
dc.contributor.authorSt John, James A
dc.contributor.authorNazareth, Lynn
dc.contributor.authorEkberg, Jenny AK
dc.date.accessioned2019-05-29T12:37:40Z
dc.date.available2019-05-29T12:37:40Z
dc.date.issued2018
dc.identifier.issn1044-7431
dc.identifier.doi10.1016/j.mcn.2018.01.001
dc.identifier.urihttp://hdl.handle.net/10072/380783
dc.description.abstractLamellipodia in Schwann cells (SCs) are crucial for myelination, but their other biological functions remain largely uncharacterised. Two types of lamellipodia exist in SCs: axial lamellipodia at the outermost edge of the cell processes, and radial lamellipodia appearing peripherally along the entire cell. We have previously shown that radial lamellipodia on olfactory glia (olfactory ensheathing cells; OECs) promote cell-cell adhesion, contact-mediated migration and phagocytosis. Here we have investigated whether lamellipodia in SCs have similar roles. Using live-cell imaging, we show that the radial lamellipodia in SCs are highly motile, appear at multiple cellular sites and rapidly move in a wave-like manner. We found that axial and radial lamellipodia had strikingly different roles and are regulated by different intracellular pathways. Axial lamellipodia initiated interactions with other SCs and with neurons by contacting radial lamellipodia on SCs, and budding neurites/axons. Most SC-SC interactions resulted in repulsion, and, lamellipodial activity (unlike in OECs) did not promote contact-mediated migration. We show that lamellipodia are crucial for SC-mediated phagocytosis of both axonal debris and bacteria, and demonstrated that inhibition of lamellipodial activity by blocking the Rho/Rac pathways also inhibits phagocytosis. We also show that heregulin, which induces SC differentiation and maturation, alters lamellipodial behaviour but does not affect phagocytic activity. Overall, the results show that SC lamellipodia are important for cell interactions and phagocytosis.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherElsevier
dc.publisher.placeUnited States
dc.relation.ispartofpagefrom189
dc.relation.ispartofpageto200
dc.relation.ispartofjournalMolecular and Cellular Neuroscience
dc.relation.ispartofvolume88
dc.subject.fieldofresearchNeurosciences not elsewhere classified
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchCognitive Sciences
dc.subject.fieldofresearchPsychology
dc.subject.fieldofresearchcode110999
dc.subject.fieldofresearchcode1109
dc.subject.fieldofresearchcode1702
dc.subject.fieldofresearchcode1701
dc.titleSchwann cell lamellipodia regulate cell-cell interactions and phagocytosis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.description.versionPost-print
gro.facultyGriffith Sciences, Griffith Institute for Drug Discovery
gro.rights.copyright© 2018 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorSt John, James A.
gro.griffith.authorEkberg, Jenny A.
gro.griffith.authorTello Velasquez, Johana P.
gro.griffith.authorNazareth, Lynn L.


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