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dc.contributor.authorBhattacharjee, Ripon
dc.contributor.authorMoriam, Sofia
dc.contributor.authorUmer, Muhammad
dc.contributor.authorNam-Trung, Nguyen
dc.contributor.authorShiddiky, Muhammad JA
dc.date.accessioned2019-06-19T13:08:34Z
dc.date.available2019-06-19T13:08:34Z
dc.date.issued2018
dc.identifier.issn0003-2654
dc.identifier.doi10.1039/C8AN01348A
dc.identifier.urihttp://hdl.handle.net/10072/380797
dc.description.abstractDNA methylation is one of the significant epigenetic modifications involved in mammalian development as well as in the initiation and progression of various diseases like cancer. Over the past few decades, an enormous amount of research has been carried out for the quantification of DNA methylation in the mammalian genome. Earlier, most of these methodologies used bisulfite treatment. However, the low conversion, false reading, longer assay time and complex chemical reaction are the common limitations of this method that hinder their application in routine clinical screening. Thus, as an alternative to bisulfite conversion-based DNA methylation detection, numerous bisulfite-free methods have been proposed. In this regard, electrochemical biosensors have gained much attention in recent years for being highly sensitive yet cost-effective, portable, and simple to operate. On the other hand, biosensors with optical readouts enable direct real time detection of biological molecules and are easily adaptable to multiplexing. Incorporation of electrochemical and optical readouts into bisulfite free DNA methylation analysis is paving the way for the translation of this important biomarker into standard patient care. In this review, we provide a critical overview of recent advances in the development of electrochemical and optical readout based bisulfite free DNA methylation assays.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherRoyal Society of Chemistry (RSC) Publications
dc.publisher.placeUnited Kingdom
dc.relation.ispartofpagefrom4802
dc.relation.ispartofpageto4818
dc.relation.ispartofissue20
dc.relation.ispartofjournalAnalyst
dc.relation.ispartofvolume143
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchOther chemical sciences not elsewhere classified
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode349999
dc.titleDNA methylation detection: recent developments in bisulfite free electrochemical and optical approaches
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, School of Environment and Science
gro.hasfulltextNo Full Text
gro.griffith.authorNguyen, Nam-Trung
gro.griffith.authorShiddiky, Muhammad J.
gro.griffith.authorUmer, Muhammad


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