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dc.contributor.authorIslam, F
dc.contributor.authorTang, JC
dc.contributor.authorGopalan, V
dc.contributor.authorLam, AK
dc.contributor.editorAlfred K. Lam
dc.date.accessioned2019-05-29T13:03:06Z
dc.date.available2019-05-29T13:03:06Z
dc.date.issued2018
dc.identifier.isbn9781493977338
dc.identifier.doi10.1007/978-1-4939-7734-5_21
dc.identifier.urihttp://hdl.handle.net/10072/380884
dc.description.abstractThe aberrant DNA methylation has been noted to occur at promoter of tumor suppressor, cell adhesion, DNA repair, and other growth regulating genes during the progression of nonneoplastic esophageal mucosa to Barrett esophagus to esophageal adenocarcinoma. Methylation-mediated silencing of individual gene or concurrent loss of a number of genes plays crucial roles in dysplasia-metaplasia-neoplasia sequence of esophageal adenocarcinoma. In addition, promoter methylation of genes had shown significant prognostic potential in patients with esophageal adenocarcinoma. Thus, determination of methylation status of genes of interest can be used as a molecular marker for risk stratification and/or better prognosis of patients with esophageal adenocarcinoma. There are a number of methods including bead array, PCR and sequencing, pyrosequencing, methylation-specific PCR, and PCR with high-resolution melt curve available to determine the methylation status of particular gene of interest. Herein, we describe the polymerase chain reaction followed by sequencing-based protocol for identifying DNA methylation status in esophageal adenocarcinoma.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer Science+Business Media, LLC
dc.publisher.placeUnited States
dc.relation.ispartofbooktitleEsophageal Adenocarcinoma: Methods and Protocols
dc.relation.ispartofchapter21
dc.relation.ispartofchapternumbers24
dc.relation.ispartofpagefrom247
dc.relation.ispartofpageto256
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchMedicinal and biomolecular chemistry
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3404
dc.titleEpigenetics: DNA Methylation Analysis in Esophageal Adenocarcinoma
dc.typeBook chapter
dc.type.descriptionB1 - Chapters
dc.type.codeB - Book Chapters
gro.facultyGriffith Health, School of Medicine
gro.hasfulltextNo Full Text
gro.griffith.authorLam, Alfred K.
gro.griffith.authorGopalan, Vinod
gro.griffith.authorIslam, Farhad


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