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  • RNAi Targeting of Human Metapneumovirus P and N Genes Inhibits Viral Growth

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    NitschinskPUB5828.pdf (199.8Kb)
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    Accepted Manuscript (AM)
    Author(s)
    Nitschinsk, Kathleen M
    Clarke, Daniel T
    Idris, Adi
    McMillan, Nigel A
    Griffith University Author(s)
    McMillan, Nigel
    Year published
    2018
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    Abstract
    Background/Aims: Human metapneumovirus (hMPV) is an important human respiratory pathogen and is implicated in an array of respiratory illnesses, ranging from asymptomatic infection to severe bronchiolitis. Currently, there is no reliable vaccine or specific antiviral therapy for hMPV infection and treatment is supportive. The use of ribonucleic acid interference has the potential to change that with the targeting of essential viral genes via small interfering RNAs (siRNAs) offering the ability to directly and rapidly treat viral infections. Method: The human lung carcinoma epithelial cell line, A549, was transfected with ...
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    Background/Aims: Human metapneumovirus (hMPV) is an important human respiratory pathogen and is implicated in an array of respiratory illnesses, ranging from asymptomatic infection to severe bronchiolitis. Currently, there is no reliable vaccine or specific antiviral therapy for hMPV infection and treatment is supportive. The use of ribonucleic acid interference has the potential to change that with the targeting of essential viral genes via small interfering RNAs (siRNAs) offering the ability to directly and rapidly treat viral infections. Method: The human lung carcinoma epithelial cell line, A549, was transfected with siRNAs targeting the N and P genes before infecting with hMPV A2 CAN97-83. Viral growth inhibition was then measured by the viral plaque assay and nucleoprotein (N) and phosphoprotein (P) gene knockdown was determined by real-time PCR. Results: In vitro prophylactic use of siRNAs targeting the 3′-abundantly expressed N and P genes of hMPV resulted in potent, sequence-specific viral inhibition. The viral inhibition was specific and not mediated by an anti-viral interferon-β response or cell death. Conclusion: The findings presented here confirmed the highly potent, sequence-specific antiviral effect of siRNAs targeting the N and P gene of hMPV. These results may facilitate the development of a novel therapeutic agent for hMPV control.
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    Journal Title
    Intervirology
    DOI
    https://doi.org/10.1159/000491927
    Copyright Statement
    © 2018 S. Karger AG, Basel. This is the peer-reviewed but unedited manuscript version of the following article: RNAi Targeting of Human Metapneumovirus P and N Genes Inhibits Viral Growth, Intervirology, 2018;61:149–154. The final, published version is available at https://doi.org/10.1159/000491927
    Subject
    Microbiology
    Virology
    Medical microbiology
    Publication URI
    http://hdl.handle.net/10072/381261
    Collection
    • Journal articles

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