Show simple item record

dc.contributor.authorNitschinsk, Kathleen M
dc.contributor.authorClarke, Daniel T
dc.contributor.authorIdris, Adi
dc.contributor.authorMcMillan, Nigel A
dc.date.accessioned2019-07-13T12:30:29Z
dc.date.available2019-07-13T12:30:29Z
dc.date.issued2018
dc.identifier.issn0300-5526
dc.identifier.doi10.1159/000491927
dc.identifier.urihttp://hdl.handle.net/10072/381261
dc.description.abstractBackground/Aims: Human metapneumovirus (hMPV) is an important human respiratory pathogen and is implicated in an array of respiratory illnesses, ranging from asymptomatic infection to severe bronchiolitis. Currently, there is no reliable vaccine or specific antiviral therapy for hMPV infection and treatment is supportive. The use of ribonucleic acid interference has the potential to change that with the targeting of essential viral genes via small interfering RNAs (siRNAs) offering the ability to directly and rapidly treat viral infections. Method: The human lung carcinoma epithelial cell line, A549, was transfected with siRNAs targeting the N and P genes before infecting with hMPV A2 CAN97-83. Viral growth inhibition was then measured by the viral plaque assay and nucleoprotein (N) and phosphoprotein (P) gene knockdown was determined by real-time PCR. Results: In vitro prophylactic use of siRNAs targeting the 3′-abundantly expressed N and P genes of hMPV resulted in potent, sequence-specific viral inhibition. The viral inhibition was specific and not mediated by an anti-viral interferon-β response or cell death. Conclusion: The findings presented here confirmed the highly potent, sequence-specific antiviral effect of siRNAs targeting the N and P gene of hMPV. These results may facilitate the development of a novel therapeutic agent for hMPV control.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherS. Karger AG
dc.publisher.placeSwitzerland
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto6
dc.relation.ispartofjournalIntervirology
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchVirology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode310706
dc.subject.fieldofresearchcode3207
dc.titleRNAi Targeting of Human Metapneumovirus P and N Genes Inhibits Viral Growth
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.facultyGriffith Health, School of Medical Science
gro.rights.copyright© 2018 S. Karger AG, Basel. This is the peer-reviewed but unedited manuscript version of the following article: RNAi Targeting of Human Metapneumovirus P and N Genes Inhibits Viral Growth, Intervirology, 2018;61:149–154. The final, published version is available at https://doi.org/10.1159/000491927
gro.hasfulltextFull Text
gro.griffith.authorMcMillan, Nigel


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record